ArticleThe Journal of cell biology2022
Quality assessment in light microscopy for routine use through simple tools and robust metrics.
Article in The Journal of cell biology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
26 citing papers in PubMed.
- Full Vectorial Field Sensing Using Liquid Crystal Droplet Arrays.Advanced materials (Deerfield Beach, Fla.) · 2026Article
- Monitoring microscope performance in an imaging facility using OMERO-metrics.bioRxiv : the preprint server for biology · 2026Article
- A cryo-CLEM-guided workflow for quantitative assessment and optimization of cryo-ET specimen quality.Molecules and cells · 2026Article
- Generation of In Vivo-Inspired 3D Collagen Models for Guided Tumor Invasion In Vitro.Current protocols · 2026Article
- Article
- SlimVar for rapid in vivo single-molecule tracking of chromatin regulators in plants.Nature communications · 2025Article
- Standardized measurements for monitoring and comparing multiphoton microscope systems.Nature protocols · 2025Review
- Morphological profiling data resource enables prediction of chemical compound properties.iScience · 2025Article
- Closing the multichannel gap through computational reconstruction of interaction in super-resolution microscopy.Patterns (New York, N.Y.) · 2025Review
- Evaluation of image analysis tools for the measurement of cellular morphology.Frontiers in cell and developmental biology · 2025Review
- Pixelation with concentration-encoded effective photons for quantitative molecular optical sectioning microscopy.Laser & photonics reviews · 2024Article
- Leaves to Measure Light Intensity.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Universal inverse modeling of point spread functions for SMLM localization and microscope characterization.Nature methods · 2024Article
- Benchtop mesoSPIM: a next-generation open-source light-sheet microscope for cleared samples.Nature communications · 2024Article
- Community-developed checklists for publishing images and image analyses.Nature methods · 2024Review
- "MeiQuant": An Integrated Tool for Analyzing Meiotic Prophase I Spread Images.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Development of AI-assisted microscopy frameworks through realistic simulation with pySTED.Nature machine intelligence · 2024Article
- Quantitatively mapping local quality of super-resolution microscopy by rolling Fourier ring correlation.Light, science & applications · 2023Article
- The Benchtop mesoSPIM: a next-generation open-source light-sheet microscope for large cleared samples.bioRxiv : the preprint server for biology · 2023Article
- Editorial: Histochemistry and Cell Biology implements new submission guidelines for image presentation and image analysis.Histochemistry and cell biology · 2023Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
13 authors.
Funding
Abstract
Although there is a need to demonstrate reproducibility in light microscopy acquisitions, the lack of standardized guidelines monitoring microscope health status over time has so far impaired the widespread use of quality control (QC) measurements. As scientists from 10 imaging core facilities who encounter various types of projects, we provide affordable hardware and open source software tools, rigorous protocols, and define reference values to assess QC metrics for the most common fluorescence light microscopy modalities. Seven protocols specify metrics on the microscope resolution, field illumination flatness, chromatic aberrations, illumination power stability, stage drift, positioning repeatability, and spatial-temporal noise of camera sensors. We designed the MetroloJ_QC ImageJ/Fiji Java plugin to incorporate the metrics and automate analysis. Measurements allow us to propose an extensive characterization of the QC procedures that can be used by any seasoned microscope user, from research biologists with a specialized interest in fluorescence light microscopy through to core facility staff, to ensure reproducible and quantifiable microscopy results.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.