ArticleProtein science : a publication of the Protein Society2022
Stabilizing proteins, simplified: A Rosetta-based webtool for predicting favorable mutations.
Article in Protein science : a publication of the Protein Society, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Intensity modulation of trichromatic split fluorescent proteins for live cell mapping.Cell reports methods · 2026Article
- Computational design of orthogonal TCR α/β interfaces for dual-TCR therapeutics.bioRxiv : the preprint server for biology · 2026Article
- Integrated computation-guided thermostabilization of the fungal sweet protein Honey Truffle Sweetener.Current research in food science · 2026Article
- AFrontiers in immunology · 2026Article
- Characterization and immunogenicity of nanoparticle vaccines based on Clade 2 and Clade 3 sarbecovirus S2 proteins.NPJ vaccines · 2025Article
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- AlphaFold modeling uncovers global structural features of class I and class II fungal hydrophobins.Protein science : a publication of the Protein Society · 2025Article
- Enhanced l-serine synthesis inSynthetic and systems biotechnology · 2025Article
- The role of multivalency in the association of the eight twenty-one protein 2 (ETO2) with the nucleosome remodeling and deacetylase (NuRD) complex.Nucleic acids research · 2025Article
- Molecular and functional profiling of Gαi as an intracellular pH sensor.Nature communications · 2025Article
- Multi-strategy ugt mining, modification and glycosyl donor synthesis facilitate the production of triterpenoid saponins.Frontiers in plant science · 2025Review
- Protein stability models fail to capture epistatic interactions of double point mutations.Protein science : a publication of the Protein Society · 2025Article
- Quantifying Protein-Nucleic Acid Interactions for Engineering Useful CRISPR-Cas9 Genome-Editing Variants.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Protein stability models fail to capture epistatic interactions of double point mutations.bioRxiv : the preprint server for biology · 2024Article
- An integrative approach to protein sequence design through multiobjective optimization.PLoS computational biology · 2024Article
- High-throughput system for the thermostability analysis of proteins.Protein science : a publication of the Protein Society · 2024Article
- Transfer learning to leverage larger datasets for improved prediction of protein stability changes.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Transfer learning to leverage larger datasets for improved prediction of protein stability changes.bioRxiv : the preprint server for biology · 2023Article
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6 authors.
Funding
Abstract
Many proteins have low thermodynamic stability, which can lead to low expression yields and limit functionality in research, industrial and clinical settings. This article introduces two, web-based tools that use the high-resolution structure of a protein along with the Rosetta molecular modeling program to predict stabilizing mutations. The protocols were recently applied to three genetically and structurally distinct proteins and successfully predicted mutations that improved thermal stability and/or protein yield. In all three cases, combining the stabilizing mutations raised the protein unfolding temperatures by more than 20°C. The first protocol evaluates point mutations and can generate a site saturation mutagenesis heatmap. The second identifies mutation clusters around user-defined positions. Both applications only require a protein structure and are particularly valuable when a deep multiple sequence alignment is not available. These tools were created to simplify protein engineering and enable research that would otherwise be infeasible due to poor expression and stability of the native molecule.
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