Evidence map›Paper›PMID 36173169›Full record

ArticleProtein science : a publication of the Protein Society2022

A genetically encoded far-red fluorescent calcium ion biosensor derived from a biliverdin-binding protein.

Rina Hashizume, Hajime Fujii, Sohum Mehta, Keisuke Ota, Yong Qian, Wenchao Zhu, Mikhail Drobizhev, Yusuke Nasu, Jin Zhang, Haruhiko Bito and 1 more

Abstract read
In one paragraph

Article in Protein science : a publication of the Protein Society, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed.

  1. Genetically encoded sensors illuminateIBRO neuroscience reports · 2025
    Article
  2. Article
  3. Two-Photon Microscopy to Measure Calcium Signaling in the Living Brain.Methods in molecular biology (Clifton, N.J.) · 2025
    Article
  4. Article
  5. Article
  6. Review
  7. Review
  8. Review
  9. Article
  10. Review
  11. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Rina HashizumeDepartment of Chemistry, School of Science, The University of Tokyo, Bunkyo-ku, Tokyo, Japan.
Hajime FujiiDepartment of Neurochemistry, Graduate School of Medicine, The University of Tokyo, Bunkyo-ku, Tokyo, Japan.
Sohum MehtaDepartment of Pharmacology, University of California San Diego, La Jolla, California, USA.ORCID 0000-0003-4764-8579
Keisuke OtaDepartment of Neurochemistry, Graduate School of Medicine, The University of Tokyo, Bunkyo-ku, Tokyo, Japan.
Yong QianDepartment of Chemistry, University of Alberta, Edmonton, Alberta, Canada.ORCID 0000-0001-7849-9022
Wenchao ZhuDepartment of Chemistry, School of Science, The University of Tokyo, Bunkyo-ku, Tokyo, Japan.
Mikhail DrobizhevDepartment of Microbiology and Cell Biology, Montana State University, Bozeman, Montana, USA.
Yusuke NasuDepartment of Chemistry, School of Science, The University of Tokyo, Bunkyo-ku, Tokyo, Japan.ORCID 0000-0002-3193-9104
Jin ZhangDepartment of Pharmacology, University of California San Diego, La Jolla, California, USA.ORCID 0000-0001-7145-7823
Haruhiko BitoDepartment of Neurochemistry, Graduate School of Medicine, The University of Tokyo, Bunkyo-ku, Tokyo, Japan.ORCID 0000-0001-6315-9594
Robert E CampbellDepartment of Chemistry, School of Science, The University of Tokyo, Bunkyo-ku, Tokyo, Japan.ORCID 0000-0003-0604-092X

Funding

Mechanisms of Compartmentalized cAMP SignalingR01DK073368 · NIDDK · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI Jin Zhang · 2006 to 2026
$7.6M
Resource for Multiphoton Characterization of Genetically-Encoded ProbesU24NS109107 · NINDS · MONTANA STATE UNIVERSITY - BOZEMAN · PI Mikhail Drobizhev · 2018 to 2026
$2.2M
CIHRNIDDK NIH HHS R01 DK073368NINDS NIH HHS U24 NS109107
6 · The paper itself

Abstract

Far-red and near-infrared (NIR) genetically encoded calcium ion (Ca

Indexed as

BiliverdineBiosensing TechniquesAnimalsCalciumCarrier ProteinsHEK293 CellsHumansIonsLuminescent ProteinsMiceBiliverdineCalciumCarrier ProteinsIonsLuminescent Proteinsbacterial phytochrome-derived fluorescent proteinscalcium ion imagingfar-red fluorescenceprotein engineering

Identifiers

PMID36173169
PMCPMC9518226

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.