ArticleBMC veterinary research2022
Validation of an automated immunoturbidimetric assay for feline serum amyloid A.
Article in BMC veterinary research, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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13 citing papers in PubMed, 14 citations in OpenAlex.
- Increased serum amyloid A and urinary kidney injury marker-1 concentrations in cats with pyelonephritis: a diagnostic accuracy study.The Journal of small animal practice · 2026Article
- Analytical comparison of two commercially available feline serum amyloid A assays using residual clinical plasma samples.The Journal of veterinary medical science · 2026Article
- Clinical and sero-diagnosis of feline leptosprosis in the sleman region of Yogyakarta, Indonesia.Open veterinary journal · 2026Article
- Association between PCV and degree of azotemia with serum hepcidin concentration in cats with chronic kidney disease.Journal of veterinary internal medicine · 2026Article
- Serum amyloid A reference intervals in raccoon dogs (Nyctereutes procyonoides koreensis): Implications for detecting inflammation.The Journal of veterinary medical science · 2025Article
- Comparison of outcomes between laparotomic and laparoscopic elective ovariectomy in anestrus dogs: postoperative recovery, pain and inflammatory biomarkers.Veterinary research communications · 2025Article
- The Value of Neutrophil Cell Population Data Parameters as Markers of Systemic Inflammation in Dogs and Cats.Veterinary clinical pathology · 2025Article
- Effect of compound polysaccharide on immunity, antioxidant capacity, gut microbiota, and serum metabolome in kittens.Frontiers in microbiology · 2025Article
- The Diagnostic Value of Serum Amyloid A and Other Laboratory and Clinical Variables in Cats with Increased Liver Enzyme Activity.Veterinary sciences · 2024Article
- Measurement of Feline Alpha-1 Acid Glycoprotein in Serum and Effusion Using an ELISA Method: Analytical Validation and Diagnostic Role for Feline Infectious Peritonitis.Pathogens (Basel, Switzerland) · 2024Article
- Article
- Effects of prednisolone on 1,2-O-dilauryl-rac-glycero glutaric acid-(60-methylresorufin) ester-lipase activity and pancreatic lipase immunoreactivity in healthy cats.Journal of veterinary internal medicineArticle
- Usefulness of serum amyloid A for the diagnosis of pyelonephritis in cats: A prospective evaluation.Journal of veterinary internal medicineArticle
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundSerum Amyloid A (SAA) is a major acute phase protein in cats, increasing rapidly in response to various inflammatory diseases. An automated latex-enhanced immunoturbidimetric assay for human SAA (LZ-SAA, Eiken), previously validated for use in cats, has had further major modification (VET-SAA, Eiken) for specific use in veterinary diagnostic laboratories but has yet to be validated in cats.
resultsIntra-assay and inter-assay CVs for the VET-SAA assay ranged from 1.88-3.57% and 3.98-6.74%, respectively. Linearity under dilution was acceptable with no prozone effect observed. Limit of detection was 1.65 mg/L and limit of quantification was 6 mg/L. Haemoglobin and triglyceride showed no adverse interference, but bilirubin produced positive bias in samples with low SAA. Comparison with the LZ-SAA assay showed significant correlation with proportional bias increasing as SAA concentration increased, likely related to differing calibration standards. SAA was significantly higher in patients with inflammatory disease compared with non-inflammatory disease, and in patients with moderate to highly elevated α1-AGP compared with patients with normal α1-AGP. Improvement of the assay range may be required to fully evaluate differences between disease groups at low SAA levels. Based on ROC curve analysis, at a cut-off point of 20.1 mg/L the VET-SAA assay discriminated between inflammatory and non-inflammatory disease with sensitivity of 0.93 and specificity of 0.99.
conclusionsThe automated VET-SAA assay is a robust, precise, and accurate method for measurement of feline SAA which can clearly identify patients with inflammatory disease. It should be a valuable biomarker for use in feline medicine.
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