Evidence map›Paper›PMID 36169860›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2023

Detecting Monoamine Oxidase A and B Proteins: A Western Blotting Protocol and Some Practical Considerations.

Jennifer N K Nyarko, Ryan M Heistad, Paul R Pennington, Darrell D Mousseau

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Jennifer N K NyarkoDepartment of Psychiatry, University of Saskatchewan, Saskatoon, SK, Canada.
Ryan M HeistadDepartment of Psychiatry, University of Saskatchewan, Saskatoon, SK, Canada.
Paul R PenningtonDepartment of Psychiatry, University of Saskatchewan, Saskatoon, SK, Canada.
Darrell D MousseauDepartment of Psychiatry, University of Saskatchewan, Saskatoon, SK, Canada. darrell.mousseau@usask.ca.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The expression of the two isoforms of monoamine oxidase (MAO A and MAO B) is often inferred from proxy measures such as mRNA transcript levels or catalytic activity. Yet the literature is clear that the proportionality of protein, mRNA, and activity does not guarantee that any of these measures can be used as a proxy for any of the others. Here we provide a protocol for the detection of MAO proteins in cell lysates that can be adapted readily to tissue preparations. Given that MAOs influence many physiological and pathological processes, we feel it is essential to include measures of protein expression when exploring genetic regulation or catalytic properties of these important enzymes.

Indexed as

Monoamine OxidaseMonoamine Oxidase InhibitorsBlotting, WesternProtein IsoformsRNA, MessengerMonoamine OxidaseMonoamine Oxidase InhibitorsProtein IsoformsRNA, MessengerCell type-specific MAO expressionMAO antibodyProtein detectionSDS-stable MAO dimerWestern blot

Identifiers

PMID36169860

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.