Evidence map›Paper›PMID 36169675›Full record

ArticleAnalytical and bioanalytical chemistry2022

Application of the aqueous two-phase system and nanozyme signal enhancement for the improved detection of Plasmodium lactate dehydrogenase in serum.

Frances D Nicklen, Alexia J Diaz, Jiakun Lu, Salil T Patel, Elaine M Zheng, Veronica R Campbell, Benjamin M Wu, Daniel T Kamei

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Article in Analytical and bioanalytical chemistry, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.5field-weighted citation impact, top 46% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 7 citations in OpenAlex.

  1. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 1 institution in 1 country.

Frances D Nicklen *Department of Bioengineering, University of California, Los Angeles, CA, 90095, USA.
Alexia J Diaz *Department of Bioengineering, University of California, Los Angeles, CA, 90095, USA.
Jiakun Lu *Department of Bioengineering, University of California, Los Angeles, CA, 90095, USA.
Salil T Patel *Department of Bioengineering, University of California, Los Angeles, CA, 90095, USA.
Elaine M ZhengDepartment of Bioengineering, University of California, Los Angeles, CA, 90095, USA.
Veronica R CampbellDepartment of Bioengineering, University of California, Los Angeles, CA, 90095, USA.
Benjamin M WuDepartment of Bioengineering, University of California, Los Angeles, CA, 90095, USA.
Daniel T KameiDepartment of Bioengineering, University of California, Los Angeles, CA, 90095, USA. kamei@seas.ucla.edu.
University of California, Los Angeles · US

Funding

National Science Foundation 1707194
6 · The paper itself

Abstract

Malaria is an infectious disease that can cause severe sickness and death if not diagnosed and treated in a timely manner. The current gold standard technique for malaria diagnosis is microscopy, which requires a dedicated laboratory setting and trained personnel and can have a long time to result. These requirements can be alleviated using paper-based diagnostic devices that enable rapid and inexpensive diagnosis at the point of care, which can allow patients to receive treatment before their symptoms progress when used for early detection of diseases. The lateral-flow immunoassay (LFA) is one such device, but currently available LFAs are susceptible to false negative results caused by low parasite density. To improve sensitivity and detection, we utilized the aqueous two-phase system (ATPS) to concentrate and purify the sample, and nanozyme signal enhancement to increase the intensity of the visible signal on the test strip. We were able to achieve a limit of detection (LOD) of 0.01 ng/mL for the malaria biomarker Plasmodium lactate dehydrogenase (pLDH) in human serum using a multi-step assay combining the LFA format with the ATPS and nanozyme signal enhancement.

Indexed as

MalariaPlasmodiumHumansImmunoassayLimit of DetectionL-Lactate DehydrogenaseL-Lactate DehydrogenaseAqueous two-phase system (ATPS) preconcentrationLateral-flow immunoassay (LFA)MalariaNanozyme signal enhancementPlatinum-coated gold nanozyme probes (PtGNP)Point-of-care diagnostics

Identifiers

PMID36169675
OpenAlexW4297458006

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.