ArticleMolecular plant pathology2023
Molecular characterization reveals no functional evidence for naturally occurring cross-kingdom RNA interference in the early stages of Botrytis cinerea-tomato interaction.
Article in Molecular plant pathology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
21 citing papers in PubMed, 35 citations in OpenAlex.
- Nanomaterial-mediated delivery of tomato miR393 intrinsically targeting a cell wall-related gene of Botrytis cinerea confers efficient control of gray mold disease.Plant communications · 2026Article
- Loss of virulence ofmBio · 2026Article
- Next-Generation Biopesticides for the Control of Fungal Plant Pathogens.Plants (Basel, Switzerland) · 2026Review
- RNA Interference in Plant Interactions with Pathogenic Microorganisms: A Weapon or a Liability?Current issues in molecular biology · 2025Review
- RNA Interference in Fungal Plant Pathogens: What Do We Know fromJournal of fungi (Basel, Switzerland) · 2025Review
- Genome-wide identification of long non-coding RNA for Botrytis cinerea during infection to tomato (Solanum lycopersicum) leaves.BMC genomics · 2025Article
- Clade-wide fungal proteome analysis reveals structure-function conservation in divergent Dicer proteins.Computational and structural biotechnology journal · 2025Article
- Broad-scale phenotyping in Arabidopsis reveals varied involvement of RNA interference across diverse plant-microbe interactions.Plant direct · 2024Article
- Mycologists and Virologists Align: ProposingViruses · 2024Review
- Botrytis cinerea combines four molecular strategies to tolerate membrane-permeating plant compounds and to increase virulence.Nature communications · 2024Article
- Genome Comparisons betweenJournal of fungi (Basel, Switzerland) · 2024Article
- Codon Optimization Enables the Geneticin Resistance Gene to Be Applied Efficiently to the Genetic Manipulation of the Plant Pathogenic FungusPlants (Basel, Switzerland) · 2024Article
- A fungal RNA-dependent RNA polymerase is a novel player in plant infection and cross-kingdom RNA interference.PLoS pathogens · 2023Article
- A perspective on cross-kingdom RNA interference in mutualistic symbioses.The New phytologist · 2023Review
- RNA-Based Control of Fungal Pathogens in Plants.International journal of molecular sciences · 2023Review
- Interactions and Insertion ofInternational journal of molecular sciences · 2023Article
- Site-specific analysis reveals candidate cross-kingdom small RNAs, tRNA and rRNA fragments, and signs of fungal RNA phasing in the barley-powdery mildew interaction.Molecular plant pathology · 2023Article
- Corrigendum.Molecular plant pathology · 2023Article
- Molecular characterization of cross-kingdom RNA interference inFrontiers in plant science · 2023Article
- Fungal biotechnology: From yesterday to tomorrow.Frontiers in fungal biology · 2023Review
Corrections and comments
- Erratum issuedCorrigendum.2023
Authors and funding
12 authors at 4 institutions in 5 countries.
Funding
Abstract
Plant immune responses are triggered during the interaction with pathogens. The fungus Botrytis cinerea has previously been reported to use small RNAs (sRNAs) as effector molecules capable of interfering with the host immune response. Conversely, a host plant produces sRNAs that may interfere with the infection mechanism of an intruder. We used high-throughput sequencing to identify sRNAs produced by B. cinerea and Solanum lycopersicum (tomato) during early phases of interaction and to examine the expression of their predicted mRNA targets in the other organism. A total of 7042 B. cinerea sRNAs were predicted to target 3185 mRNAs in tomato. Of the predicted tomato target genes, 163 were indeed transcriptionally down-regulated during the early phase of infection. Several experiments were performed to study a causal relation between the production of B. cinerea sRNAs and the down-regulation of predicted target genes in tomato. We generated B. cinerea mutants in which a transposon region was deleted that is the source of c.10% of the fungal sRNAs. Furthermore, mutants were generated in which both Dicer-like genes (Bcdcl1 and Bcdcl2) were deleted and these displayed a >99% reduction of transposon-derived sRNA production. Neither of these mutants was significantly reduced in virulence on any plant species tested. Our results reveal no evidence for any detectable role of B. cinerea sRNAs in the virulence of the fungus.
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Registered trials
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