ArticlePlant biotechnology journal2023
Metabolic engineering of p-hydroxybenzoate in poplar lignin.
Article in Plant biotechnology journal, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- The Plant Cell Wall: From Structural Diversity and Biosynthesis to Integrity Signaling and Physiological Functions.Plants (Basel, Switzerland) · 2026Review
- NMR of Fully and PartiallyAnalytical chemistry · 2026Article
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- KNAT7 transcription factor regulates metabolite and ion profiles to control cell wall biosynthesis in Populus.Scientific reports · 2026Article
- Article
- Physiological roles of lignins - tuning cell wall hygroscopy and biomechanics.The New phytologist · 2025Review
- Lignin Biosynthesis Driven byPlants (Basel, Switzerland) · 2025Article
- Chromatic symphony of fleshy fruits: functions, biosynthesis and metabolic engineering of bioactive compounds.Molecular horticulture · 2025Review
- Suppression of Chorismate Mutase 1 in Hybrid Poplar to Investigate Potential Redundancy in the Supply of Lignin Precursors.Plant direct · 2025Article
- Polyphenolic Composition, Antioxidant Activity, and Cytotoxic Effect of Male Floral Buds from ThreeMolecules (Basel, Switzerland) · 2025Article
- Article
- Synthetic-biology approach for plant lignocellulose engineering.Plant biotechnology (Tokyo, Japan) · 2024Article
- The plant cell wall-dynamic, strong, and adaptable-is a natural shapeshifter.The Plant cell · 2024Review
- Lignin Hydrogenolysis: Phenolic Monomers from Lignin and Associated Phenolates across Plant Clades.ACS sustainable chemistry & engineering · 2023Article
- Article
- Evolution of p-coumaroylated lignin in eudicots provides new tools for cell wall engineering.The New phytologist · 2023Article
- Lignin engineering in forest trees: From gene discovery to field trials.Plant communications · 2022Review
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Ester-linked p-hydroxybenzoate occurs naturally in poplar lignin as pendent groups that can be released by mild alkaline hydrolysis. These 'clip-off' phenolics can be separated from biomass and upgraded into diverse high-value bioproducts. We introduced a bacterial chorismate pyruvate lyase gene into transgenic poplar trees with the aim of producing more p-hydroxybenzoate from chorismate, itself a metabolic precursor to lignin. By driving heterologous expression specifically in the plastids of cells undergoing secondary wall formation, this strategy achieved a 50% increase in cell-wall-bound p-hydroxybenzoate in mature wood and nearly 10 times more in developing xylem relative to control trees. Comparable amounts also remained as soluble p-hydroxybenzoate-containing xylem metabolites, pointing to even greater engineering potential. Mass spectrometry imaging showed that the elevated p-hydroxybenzoylation was largely restricted to the cell walls of fibres. Finally, transgenic lines outperformed control trees in assays of saccharification potential. This study highlights the biotech potential of cell-wall-bound phenolate esters and demonstrates the importance of substrate supply in lignin engineering.
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