How to Generate a Vascular-Labelled Transgenic Zebrafish Model to Study Tumor Angiogenesis and Extravasation.

Roxana E Oberkersch, Jacopo Lidonnici, Massimo M Santoro

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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 · Who and what money

Authors and funding

3 authors.

Roxana E OberkerschLaboratory of Angiogenesis and Cancer Metabolism, Department of Biology, University of Padova, Padova, Italy.

Jacopo LidonniciLaboratory of Angiogenesis and Cancer Metabolism, Department of Biology, University of Padova, Padova, Italy.

Massimo M SantoroLaboratory of Angiogenesis and Cancer Metabolism, Department of Biology, University of Padova, Padova, Italy. massimo.santoro@unipd.it.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The use of transgenic animals carrying exogenous DNA integrated in their genome is a routine in modern-day laboratories. Nowadays, the zebrafish system represents the most useful tool for transgenesis studies mainly due to easy accessibility and manipulation of the eggs, which are produced in high numbers and over a relatively short generation time. The zebrafish transgenic technology is very straightforward when coupled with angiogenesis studies allowing easy in vivo observation of the vertebrate embryonic vasculature. Here, we describe the most common technique to generate vascular-labelled transgenic zebrafish embryos and their applications to study tumor angiogenesis and visualize tumor extravasation.

Indexed as

NeoplasmsZebrafishAnimalsAnimals, Genetically ModifiedDNANeovascularization, PathologicDNAEndothelial cellsExtravasationMural cellsTumor angiogenesisZebrafish embryos

Identifiers

PMID36161418

DOI10.1016/j.chembiol.2019.01.009

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.