ArticleFrontiers in microbiology2022
Impact of preservation method and storage period on ribosomal metabarcoding of marine microbes: Implications for remote automated samplings.
Article in Frontiers in microbiology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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Who cites it
2 citing papers in PubMed.
- Microbial community characterization in Red Sea-derived samples using a field-deployable DNA extraction system and nanopore sequencing.Environmental microbiome · 2025Article
- Seven years in formalin: the burden of metabarcoding mesozooplankton preserved in buffered formalin from the northern Adriatic Sea.Journal of plankton researchArticle
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Authors and funding
7 authors.
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Abstract
Automated sampling technologies can enhance the temporal and spatial resolution of marine microbial observations, particularly in remote and inaccessible areas. A critical aspect of automated microbiome sampling is the preservation of nucleic acids over long-term autosampler deployments. Understanding the impact of preservation method on microbial metabarcoding is essential for implementing genomic observatories into existing infrastructure, and for establishing best practices for the regional and global synthesis of data. The present study evaluates the effect of two preservatives commonly used in autosampler deployments (mercuric chloride and formalin) and two extraction kits (PowerWater and NucleoSpin) on amplicon sequencing of 16S and 18S rRNA gene over 50 weeks of sample storage. Our results suggest the combination of mercuric chloride preservation and PowerWater extraction as most adequate for 16S and 18S rRNA gene amplicon-sequencing from the same seawater sample. This approach provides consistent information on species richness, diversity and community composition in comparison to control samples (nonfixed, filtered and frozen) when stored up to 50 weeks at
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