ArticleMaterials today. Bio2022
The combination of a 3D-Printed porous Ti-6Al-4V alloy scaffold and stem cell sheet technology for the construction of biomimetic engineered bone at an ectopic site.
Article in Materials today. Bio, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Biomimetic Strategies for Bone Regeneration: Smart Scaffolds and Multiscale Cues.Biomimetics (Basel, Switzerland) · 2025Review
- Hydroxyapatite-Complexed Type I Collagen and Fibrinogen-Modified Porous Titanium Alloy Scaffold: Promoting Osteogenesis and Soft Tissue Integration.Micromachines · 2025Article
- Advanced surface modification techniques for titanium implants: a review of osteogenic and antibacterial strategies.Frontiers in bioengineering and biotechnology · 2025Review
- A multifunctional ionic liquid coating on 3D-Printed prostheses: Combating infection, promoting osseointegration.Materials today. Bio · 2024Article
- New insight into biodegradable macropore filler on tuning mechanical properties and bone tissue ingrowth in sparingly dissolvable bioceramic scaffolds.Materials today. Bio · 2024Article
- Fabrication and properties of PLA/β-TCP scaffolds using liquid crystal display (LCD) photocuring 3D printing for bone tissue engineering.Frontiers in bioengineering and biotechnology · 2024Article
- Biodegradable Implants for Internal Fixation of Fractures and Accelerated Bone Regeneration.ACS omega · 2023Review
- Metal-organic frameworks functionalized biomaterials for promoting bone repair.Materials today. Bio · 2023Review
- Isolation and identification of novel selective antitumor constituents, sidrin and sidroside, fromSaudi pharmaceutical journal : SPJ : the official publication of the Saudi Pharmaceutical Society · 2023Article
- Long Bone Defect Filling with Bioactive Degradable 3D-Implant: Experimental Study.Biomimetics (Basel, Switzerland) · 2023Article
- Improving osteoinduction and osteogenesis of Ti6Al4V alloy porous scaffold by regulating the pore structure.Frontiers in chemistry · 2023Article
- Research advances in bioactive bone graft materials and bone marrow enrichment techniques for bone defect treatment.Cell transplantationReview
- Raising the Bar: Progress in 3D-Printed Hybrid Bone Scaffolds for Clinical Applications: A Review.Cell transplantationReview
Corrections and comments
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cell sheet technology has been widely used in bone tissue engineering and regenerative medicine. However, controlling the shape and volume of large pieces of engineered bone tissue remains impossible without additional suitable scaffolds. Three-dimensional (3D) printed titanium (Ti) alloy scaffolds are mostly used as implant materials for repairing bone defects, but the unsatisfactory bioactivities of traditional Ti-based scaffolds severely limit their clinical applications. Herein, we hypothesize that the combination of bone marrow mesenchymal stem cell (BMSC) sheet technology and 3D porous Ti-6Al-4V (PT) alloy scaffolds could be used to fabricate biomimetic engineered bone. First, various concentrations of BMSCs were directly cocultured with PT scaffolds to obtain complexes of osteoblastic cell sheets and scaffolds. Then, as an experimental control, an osteoblastic BMSC sheet was prepared by continuous culturing under osteogenic conditions for 2 weeks without passaging and used to wrap the scaffolds. The BMSC sheet was composed of several layers of extracellular matrix (ECM) and a mass of BMSCs. The BMSCs exhibited excellent adherent, proliferative and osteogenic potential when cocultured with PT scaffolds, which may be attributed to the ability of the 3D microstructure of scaffolds to facilitate the biological behaviors of cells, as confirmed by the in vitro results. Moreover, the presence of BMSCs and ECM increased the angiogenic potential of PT scaffolds by the secretion of VEGF. Micro-CT and histological analysis confirmed the in vivo formation of biomimetic engineered bone when the complex of cocultured BMSCs and PT scaffolds and the scaffolds wrapped by prepared BMSC sheets were implanted subcutaneously into nude mice. Therefore, the combination of BMSC sheet technology and 3D-printed PT scaffolds could be used to construct customized biomimetic engineered bone, offering a novel and promising strategy for the precise repair of bone defects.
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