Evidence map›Paper›PMID 36156464›Full record

ArticleAdvanced science (Weinheim, Baden-Wurttemberg, Germany)2022

Visualization of the Dynamics of Invasion and Intravasation of the Bacterium That Causes Lyme Disease in a Tissue Engineered Dermal Microvessel Model.

Zhaobin Guo, Nan Zhao, Tracy D Chung, Anjan Singh, Ikshu Pandey, Linus Wang, Xinyue Gu, Aisha Ademola, Raleigh M Linville, Utpal Pal and 2 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
2.1field-weighted citation impact, top 14% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 14 citations in OpenAlex.

  1. Article
  2. Article
  3. Review
  4. Article
  5. Article
  6. Review
  7. Development of Organoids to Study Infectious Host Interactions.Methods in molecular biology (Clifton, N.J.) · 2024
    Article
  8. Article
  9. Review
  10. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 4 institutions in 1 country.

Zhaobin GuoInstitute for Nanobiotechnology, Johns Hopkins University, 3400 N Charles St, Baltimore, MD, 21218, USA.
Nan ZhaoInstitute for Nanobiotechnology, Johns Hopkins University, 3400 N Charles St, Baltimore, MD, 21218, USA.
Tracy D ChungInstitute for Nanobiotechnology, Johns Hopkins University, 3400 N Charles St, Baltimore, MD, 21218, USA.
Anjan SinghInstitute for Nanobiotechnology, Johns Hopkins University, 3400 N Charles St, Baltimore, MD, 21218, USA.
Ikshu PandeyInstitute for Nanobiotechnology, Johns Hopkins University, 3400 N Charles St, Baltimore, MD, 21218, USA.
Linus WangInstitute for Nanobiotechnology, Johns Hopkins University, 3400 N Charles St, Baltimore, MD, 21218, USA.
Xinyue GuInstitute for Nanobiotechnology, Johns Hopkins University, 3400 N Charles St, Baltimore, MD, 21218, USA.
Aisha AdemolaDepartment of Chemistry, University of South Florida, 4202 E Fowler Ave, Tampa, FL, 33620, USA.
Raleigh M LinvilleInstitute for Nanobiotechnology, Johns Hopkins University, 3400 N Charles St, Baltimore, MD, 21218, USA.
Utpal PalDepartment of Veterinary Medicine, The University of Maryland, College Park, 8075 Greenmead Dr, College Park, MD, 20740, USA.
J Stephen DumlerJoint Department of Pathology, School of Medicine, Uniformed Services University of the Health Sciences, 4301 Jones Bridge Rd, Bethesda, MD, 20814, USA.
Peter C SearsonInstitute for Nanobiotechnology, Johns Hopkins University, 3400 N Charles St, Baltimore, MD, 21218, USA.ORCID 0000-0002-5417-0828
Johns Hopkins University · USUniformed Services University of the Health Sciences · USUniversity of Maryland, College Park · USUniversity of South Florida · US

Funding

Congressionally Directed Medical Research Programs W81XWH1920045
6 · The paper itself

Abstract

Lyme disease is a tick-borne disease prevalent in North America, Europe, and Asia. Despite the accumulated knowledge from epidemiological, in vitro, and in animal studies, the understanding of dissemination of vector-borne pathogens, such as Borrelia burgdorferi (Bb), remains incomplete with several important knowledge gaps, especially related to invasion and intravasation into circulation. To elucidate the mechanistic details of these processes a tissue-engineered human dermal microvessel model is developed. Fluorescently labeled Bb are injected into the extracellular matrix (ECM) to mimic tick inoculation. High resolution, confocal imaging is performed to visualize the sub-acute phase of infection. From analysis of migration paths no evidence to support adhesin-mediated interactions between Bb and ECM components is found, suggesting that collagen fibers serve as inert obstacles to migration. Intravasation occurs at cell-cell junctions and is relatively fast, consistent with Bb swimming in ECM. In addition, it is found that Bb alone can induce endothelium activation, resulting in increased immune cell adhesion but no changes in global or local permeability. Together these results provide new insight into the minimum requirements for Bb dissemination and highlight how tissue-engineered models are complementary to animal models in visualizing dynamic processes associated with vector-borne pathogens.

Indexed as

Borrelia burgdorferiLyme DiseaseAnimalsHumansMicrovesselsModels, AnimalSkindisseminationintravasationinvasiontissue-engineeringvector-borne pathogens

Identifiers

PMID36156464
PMCPMC9762293
OpenAlexW4297192960

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.