Evidence map›Paper›PMID 36156161›Full record

ArticleApplied microbiology and biotechnology2022

Characterization of the exopolymer-producing Pseudoalteromonas sp. S8-8 from Antarctic sediment.

Carmen Rizzo, Elena Perrin, Annarita Poli, Ilaria Finore, Renato Fani, Angelina Lo Giudice

Open access · hybridAbstract read
In one paragraph

Article in Applied microbiology and biotechnology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.8field-weighted citation impact, top 26% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 6 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 4 institutions in 2 countries.

Carmen RizzoMarine Biotechnology Department, Stazione Zoologica "Anton Dohrn", Sicily Marine Centre, Villa Pace, Messina, Italy.ORCID http://orcid.org/0000-0002-4340-3833
Elena PerrinDepartment of Biology, University of Florence, Florence, Italy.ORCID http://orcid.org/0000-0001-5148-3178
Annarita PoliInstitute of Biomolecular Chemistry, National Research Council (ICB-CNR), Pozzuoli, NA), Italy.ORCID http://orcid.org/0000-0001-6465-4765
Ilaria FinoreInstitute of Biomolecular Chemistry, National Research Council (ICB-CNR), Pozzuoli, NA), Italy.ORCID http://orcid.org/0000-0003-3606-5140
Renato FaniDepartment of Biology, University of Florence, Florence, Italy.ORCID http://orcid.org/0000-0001-9114-2335
Angelina Lo GiudiceInstitute of Polar Sciences, National Research Council (CNR-ISP), Spianata San Raineri 86, 98122, Messina, Italy. angelina.logiudice@cnr.it.ORCID http://orcid.org/0000-0002-8842-083X
National Research Council · ITUniversity of Florence · ITANT Foundation Italy Onlus · ITStazione Zoologica Anton Dohrn · IT

Funding

PNRA PNRA18_00075
6 · The paper itself

Abstract

A synergistic approach using cultivation methods, chemical, and bioinformatic analyses was applied to explore the potential of Pseudoalteromonas sp. S8-8 in the production of extracellular polymeric substances (EPSs) and the possible physiological traits related to heavy metal and/or antibiotic resistance. The effects of different parameters (carbon source, carbon source concentration, temperature, pH and NaCl supplement) were tested to ensure the optimization of growth conditions for EPS production by the strain S8-8. The highest yield of EPS was obtained during growth in culture medium supplemented with glucose (final concentration 2%) and NaCl (final concentration 3%), at 15 °C and pH 7. The EPS was mainly composed of carbohydrates (35%), followed by proteins and uronic acids (2.5 and 2.77%, respectively) and showed a monosaccharidic composition of glucose: mannose: galactosamine: galactose in the relative molar proportions of 1:0.7:0.5:0.4, as showed by the HPAE-PAD analysis. The detection of specific molecular groups (sulfates and uronic acid content) supported the interesting properties of EPSs, i.e. the emulsifying and cryoprotective action, heavy metal chelation, with interesting implication in bioremediation and biomedical fields. The analysis of the genome allowed to identify a cluster of genes involved in cellulose biosynthesis, and two additional gene clusters putatively involved in EPS biosynthesis. KEY POINTS: • A cold-adapted Pseudoalteromonas strain was investigated for EPS production. • The EPS showed emulsifying, cryoprotective, and heavy metal chelation functions. • Three gene clusters putatively involved in EPS biosynthesis were evidenced by genomic insights.

Indexed as

Metals, HeavyPseudoalteromonasAntarctic RegionsCarbonCelluloseExtracellular Polymeric Substance MatrixGalactosamineGalactoseGlucoseMannosePolysaccharides, BacterialSodium ChlorideSulfatesUronic AcidsCarbonCelluloseGalactosamineGalactoseGlucoseMannoseMetals, HeavyPolysaccharides, BacterialSodium ChlorideSulfatesUronic AcidsBiotechnologyCold adaptedCryoprotectionEPSGenome

Identifiers

PMID36156161
PMCPMC9592659
OpenAlexW4297224959

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.