Evidence map›Paper›PMID 36153401›Full record

ArticleScientific reports2022

Mannan-binding lectin serine protease-2 (MASP-2) in human kidney and its relevance for proteolytic activation of the epithelial sodium channel.

Rikke Zachar, Steffen Thiel, Søren Hansen, Maiken Lumby Henriksen, Mikkel-Ole Skjoedt, Karsten Skjodt, Zohra Hamzaei, Kirsten Madsen, Lars Lund, Edith Hummler and 2 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.3field-weighted citation impact, top 48% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 3 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 5 institutions in 2 countries.

Rikke ZacharDepartment of Cardiovascular and Renal Research, Institute of Molecular Medicine, University of Southern Denmark, J.B. Winsløvsvej 21, 3, 5000, Odense, Denmark. rzachar@health.sdu.dk.
Steffen ThielDepartment of Biomedicine, Aarhus University, Aarhus, Denmark.
Søren HansenDepartment of Cancer and Inflammation, Institute of Molecular Medicine, University of Southern Denmark, Odense, Denmark.
Maiken Lumby HenriksenDepartment of Cancer and Inflammation, Institute of Molecular Medicine, University of Southern Denmark, Odense, Denmark.
Mikkel-Ole SkjoedtDepartment of Clinical Immunology, Copenhagen University Hospital, Copenhagen, Denmark.
Karsten SkjodtDepartment of Cancer and Inflammation, Institute of Molecular Medicine, University of Southern Denmark, Odense, Denmark.
Zohra HamzaeiDepartment of Cardiovascular and Renal Research, Institute of Molecular Medicine, University of Southern Denmark, J.B. Winsløvsvej 21, 3, 5000, Odense, Denmark.
Kirsten MadsenDepartment of Cardiovascular and Renal Research, Institute of Molecular Medicine, University of Southern Denmark, J.B. Winsløvsvej 21, 3, 5000, Odense, Denmark.
Lars LundUrology and Clinical Department, Odense University Hospital, Odense, Denmark.
Edith HummlerDepartment of Biomedical Sciences, University of Lausanne, Lausanne, Switzerland.
Per SvenningsenDepartment of Cardiovascular and Renal Research, Institute of Molecular Medicine, University of Southern Denmark, J.B. Winsløvsvej 21, 3, 5000, Odense, Denmark.
Boye Lagerbon JensenDepartment of Cardiovascular and Renal Research, Institute of Molecular Medicine, University of Southern Denmark, J.B. Winsløvsvej 21, 3, 5000, Odense, Denmark.
University of Southern Denmark · DKAarhus University · DKCopenhagen University Hospital · DKOdense University Hospital · DKUniversity of Lausanne · CH

Funding

Swiss National Science Foundation 31003A_182478/1
6 · The paper itself

Abstract

Proteolytic activation of the renal epithelial sodium channel (ENaC) is increased by aldosterone. The aldosterone-sensitive protease remains unidentified. In humans, elevated circulating aldosterone is associated with increased urinary extracellular vesicle (uEVs) excretion of mannan-binding lectin associated serine protease-2 (MASP-2). We hypothesized that MASP-2 is a physiologically relevant ENaC-activating protease. It was confirmed that MASP2 mRNA is abundantly present in liver but not in human and mouse kidneys. Aldosterone-stimulation of murine cortical colleting duct (mCCD) cells did not induce MASP-2 mRNA. In human kidney collecting duct, MASP-2 protein was detected in AQP2-negative/ATP6VB1-positive intercalated cells suggestive of MASP2 protein uptake. Plasma concentration of full-length MASP-2 and the short splice variant MAp19 were not changed in a cross-over intervention study in healthy humans with low (70 mmol/day) versus high (250 mmol/day) Na

Indexed as

Kidney Tubules, CollectingMannose-Binding Protein-Associated Serine ProteasesAldosteroneAmilorideAnimalsAquaporin 2Epithelial Sodium ChannelsHEK293 CellsHumansKidneyMiceRNA, MessengerSodiumAldosteroneAmilorideAquaporin 2Epithelial Sodium ChannelsMannose-Binding Protein-Associated Serine ProteasesMASP2 protein, humanMASP-2 protein, mouseRNA, MessengerSodium

Identifiers

PMID36153401
PMCPMC9509361
OpenAlexW4297183466

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.