Evidence map›Paper›PMID 36152258›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2023

Ratiometric Fluorescent Safranin-O Staining Allows the Quantification of Lignin Contents In Muro.

Oriane Morel, Corentin Spriet, Cédric Lion, Fabien Baldacci-Cresp, Garance Pontier, Marie Baucher, Christophe Biot, Simon Hawkins, Godfrey Neutelings

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Oriane MorelUniversité de Lille, CNRS, UMR 8576, UGSF - Unité de Glycobiologie Structurale et Fonctionnelle, Lille, France.
Corentin SprietUniversité de Lille, CNRS, UMR 8576, UGSF - Unité de Glycobiologie Structurale et Fonctionnelle, Lille, France.
Cédric LionUniversité de Lille, CNRS, UMR 8576, UGSF - Unité de Glycobiologie Structurale et Fonctionnelle, Lille, France.
Fabien Baldacci-CrespUniversité de Lille, CNRS, UMR 8576, UGSF - Unité de Glycobiologie Structurale et Fonctionnelle, Lille, France.
Garance PontierUniversité de Lille, CNRS, UMR 8576, UGSF - Unité de Glycobiologie Structurale et Fonctionnelle, Lille, France.
Marie BaucherLaboratoire de Biotechnologie Végétale (LBV), Université Libre de Bruxelles, Gosselies, Belgium.
Christophe BiotUniversité de Lille, CNRS, UMR 8576, UGSF - Unité de Glycobiologie Structurale et Fonctionnelle, Lille, France.
Simon HawkinsUniversité de Lille, CNRS, UMR 8576, UGSF - Unité de Glycobiologie Structurale et Fonctionnelle, Lille, France.
Godfrey NeutelingsUniversité de Lille, CNRS, UMR 8576, UGSF - Unité de Glycobiologie Structurale et Fonctionnelle, Lille, France. godfrey.neutelings@univ-lille.fr.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

In some specific vascular plant tissues, lignin can impregnate the entire cell wall to make it more rigid and hydrophobic. Different techniques have been developed in the past years to make possible the quantification of this polyphenolic polymer at the organ or tissue level, but difficulties of access to the cellular level remain. Here we describe an approach based on ratiometric emission measurements using safranin-O and the development of a macro adapted for the FIJI software, which makes it possible to quantify lignin in three different layers of the cell wall on images captured on a fluorescent confocal microscope.

Indexed as

LigninPhenazinesCell WallColoring AgentsStaining and LabelingColoring AgentsLigninPhenazinessafranine TCell wallConfocal microscopyLignin quantificationSafranin-OSegmentation

Identifiers

PMID36152258

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.