Evidence map›Paper›PMID 36152256›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2023

Specific RNA Visualization at Electron Microscopy via Terbium Citrate Vapors.

Claudio Casali, Lorena Zannino, Marco Biggiogera

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.5field-weighted citation impact, top 37% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Resolving Sub-Nuclear Architecture from Compartments to Functional Domains.International journal of molecular sciences · 2026
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Claudio CasaliDepartment of Biology and Biotechnology "Lazzaro Spallanzani", Laboratory of Cell Biology and Neurobiology, University of Pavia, Pavia, Italy. claudio.casali@unipv.it.
Lorena ZanninoDepartment of Biology and Biotechnology "Lazzaro Spallanzani", Laboratory of Cell Biology and Neurobiology, University of Pavia, Pavia, Italy.
Marco BiggiogeraDepartment of Biology and Biotechnology "Lazzaro Spallanzani", Laboratory of Cell Biology and Neurobiology, University of Pavia, Pavia, Italy.
University of Pavia · IT

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Terbium citrate staining represents the method of choice for RNA visualization at transmission electron microscopy. Because of its affinity for guanosines in RNA rather than in DNA, terbium citrate gives precise results being a selective staining. However, difficulties often arise when performing this technique, especially in crucial and delicate steps such as rinses, when it is not uncommon to excessively reduce the already feeble contrast. For these reasons, we developed a straightforward and secure approach to overcome such complications. Here we report a new method for RNA single molecule localization by means of terbium citrate vapors, viable for every type of fixation and embedding.

Indexed as

RNATerbiumCitric AcidDNAMicroscopy, ElectronCitric AcidDNARNATerbiumCytochemistryElectron microscopyRNA stainingTerbium staining

Identifiers

PMID36152256
OpenAlexW4297174478

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.