ArticleMethods in molecular biology (Clifton, N.J.)2023
Nile Red and BODIPY Staining of Lipid Droplets in Mouse Oocytes and Embryos.
Article in Methods in molecular biology (Clifton, N.J.), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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Who cites it
4 citing papers in PubMed, 5 citations in OpenAlex.
- Lipid droplets as stress-buffering organelles in cancer cell homeostasis.Communications biology · 2026Review
- Lysophosphatidylethanolamine 18:1 drives clear cell renal cell carcinoma by stabilizing SIRT6 to reprogram lipid metabolism.Signal transduction and targeted therapy · 2025Article
- Exposure to polystyrene nanoplastics induces lysosomal enlargement and lipid droplet accumulation in KGN human ovarian granulosa cells.Archives of toxicology · 2025Article
- Female-age-dependent changes in the lipid fingerprint of the mammalian oocytes.Human reproduction (Oxford, England) · 2024Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
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Abstract
Lipid droplets (LDs) are intracellular structures composed of hydrophobic lipids. Their amount in oocytes and embryos varies among the mammalian species and even among different strains of the same species. Here we describe a method to stain LDs, which can be applied to previously fixed mouse oocytes and embryos. This method is based on fluorescent dyes, Nile red and BODIPY, which allow visualization and quantification of LDs using conventional and confocal fluorescence microscopy.
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