Evidence map›Paper›PMID 36152160›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2023

Analyses and Localization of Serotonin and L-DOPA in Ocular Tissues by Imaging Mass Spectrometry.

Varun Krishnan, Sean Meehan, Colin Hayter, Sanjoy K Bhattacharya

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
1.0field-weighted citation impact, top 24% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 2 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Varun KrishnanBascom Palmer Eye Institute, Miller School of Medicine, University of Miami, Miami, FL, USA.
Sean MeehanBascom Palmer Eye Institute, Miller School of Medicine, University of Miami, Miami, FL, USA.
Colin HayterBascom Palmer Eye Institute, Miller School of Medicine, University of Miami, Miami, FL, USA.
Sanjoy K BhattacharyaBascom Palmer Eye Institute, Miller School of Medicine, University of Miami, Miami, FL, USA. sbhattacharya@med.miami.edu.
University of Miami · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Imaging mass spectrometry (IMS) allows for visualization of the spatial distribution of proteins, lipids, and other metabolites in a targeted or untargeted approach. The identification of compounds through mass spectrometry combined with the mapping of compound distribution in the sample establishes IMS as a powerful tool for metabolomics. IMS analysis for serotonin will allow researchers to pinpoint areas of deficiencies or accumulations associated with ocular disorders such as serotonin selective reuptake inhibitor optic neuropathy. Furthermore, L-DOPA has shown great promise as a therapeutic approach for disorders such as age-related macular degeneration, and IMS allows for localization, and relative magnitudes, of L-DOPA in the eye. We describe here an end-to-end approach of IMS from sample preparation to data analysis for serotonin and L-DOPA analysis.

Indexed as

LevodopaSerotoninLipidsMetabolomicsSpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationLevodopaLipidsSerotoninHigh-resolution mass spectrometryImaging mass spectrometryL-DOPALocalizationMALDIMass spectrometryMetabolite localizationMetabolite visualizationMetabolomicsSerotonin

Identifiers

PMID36152160
OpenAlexW4296982022

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.