ArticleMethods in molecular biology (Clifton, N.J.)2022
Exploring the Topology of Cytoplasmic Membrane Proteins Involved in Lipopolysaccharide Biosynthesis by in Silico and Biochemical Analyses.
Article in Methods in molecular biology (Clifton, N.J.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
In the absence of a tri-dimensional structure, revealing the topology of a membrane protein provides relevant information to identify the number and orientation of transmembrane helices and the localization of critical amino acid residues, contributing to a better understanding of function and intermolecular associations. Topology can be predicted in silico by bioinformatic analysis or solved by biochemical methods. In this chapter, we describe a pipeline employing bioinformatic approaches for the prediction of membrane protein topology, followed by experimental validation through the substituted-cysteine accessibility method and the analysis of the protein's oligomerization state.
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