ArticleNature communications2022
Cytoplasmic organization promotes protein diffusion in Xenopus extracts.
Article in Nature communications, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Exchange dynamics and kinetic control of gene regulation complexes.Nature reviews. Molecular cell biology · 2026Review
- Compartmentalized cytoplasmic tradewinds direct soluble proteins.Nature communications · 2026Article
- How does cytoplasmic crowding affect reaction rates?Molecular cell · 2026Review
- Intracellular diffusion in the cytoplasm increases with cell size in fission yeast.Molecular biology of the cell · 2025Article
- Single-molecule diffusivity quantification inProceedings of the National Academy of Sciences of the United States of America · 2024Article
- Robust trigger wave speed in Xenopus cytoplasmic extracts.Nature communications · 2024Article
- Article
- Viscosity-dependent control of protein synthesis and degradation.Nature communications · 2024Article
- Membrane localization accelerates association under conditions relevant to cellular signaling.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Metastable States in the Hinge-Bending Landscape of an Enzyme in an Atomistic Cytoplasm Simulation.The journal of physical chemistry letters · 2024Article
- Long-range formation of the Bicoid gradient requires multiple dynamic modes that spatially vary across the embryo.Development (Cambridge, England) · 2024Article
- Measuring Molecular Diffusion in Self-Organizing Xenopus Extracts by Fluorescence Correlation Spectroscopy.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Robust trigger wave speed inbioRxiv : the preprint server for biology · 2023Article
- How it feels in a cell.Trends in cell biology · 2023Review
- Protein homeostasis from diffusion-dependent control of protein synthesis and degradation.bioRxiv : the preprint server for biology · 2023Article
- Vast heterogeneity in cytoplasmic diffusion rates revealed by nanorheology and Doppelgänger simulations.Biophysical journal · 2023Article
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Authors and funding
3 authors.
Funding
Abstract
The cytoplasm is highly organized. However, the extent to which this organization influences the dynamics of cytoplasmic proteins is not well understood. Here, we use Xenopus laevis egg extracts as a model system to study diffusion dynamics in organized versus disorganized cytoplasm. Such extracts are initially homogenized and disorganized, and self-organize into cell-like units over the course of tens of minutes. Using fluorescence correlation spectroscopy, we observe that as the cytoplasm organizes, protein diffusion speeds up by about a factor of two over a length scale of a few hundred nanometers, eventually approaching the diffusion time measured in organelle-depleted cytosol. Even though the ordered cytoplasm contained organelles and cytoskeletal elements that might interfere with diffusion, the convergence of protein diffusion in the cytoplasm toward that in organelle-depleted cytosol suggests that subcellular organization maximizes protein diffusivity. The effect of organization on diffusion varies with molecular size, with the effects being largest for protein-sized molecules, and with the time scale of the measurement. These results show that cytoplasmic organization promotes the efficient diffusion of protein molecules in a densely packed environment.
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