ArticleSTAR protocols2022
Assembly of plant virus agroinfectious clones using biological material or DNA synthesis.
Article in STAR protocols, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed.
- Protocol for triggering plant gene silencing by viral delivery of short RNA.STAR protocols · 2025Article
- Characterization of an unusual carlavirus-like RNA from papaya (Carica papaya) lacking essential genes.PloS one · 2025Article
- N6-adenosine-methyltransferase MTA70-like participates antiviral responses inFrontiers in microbiology · 2025Article
- syn-tasiRnas targeting the coat protein of potato virus Y confer antiviral resistance inPlant signaling & behavior · 2024Article
- Deploying deep Solanaceae domestication and virus biotechnology knowledge to enhance food system performance and diversity.Horticulture research · 2024Article
- Methyltransferase-like (METTL) homologues participate inPlant signaling & behavior · 2023Article
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Authors and funding
1 author.
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Abstract
Infectious clone technology is universally applied for biological characterization and engineering of viruses. This protocol describes procedures that implement synthetic biology advances for streamlined assembly of virus infectious clones. Here, I detail homology-based cloning using biological material, as well as SynViP assembly using type IIS restriction enzymes and chemically synthesized DNA fragments. The assembled virus clones are based on compact T-DNA binary vectors of the pLX series and are delivered to host plants by Agrobacterium-mediated inoculation. For complete details on the use and execution of this protocol, please refer to Pasin et al. (2017, 2018) and Pasin (2021).
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