Evidence map›Paper›PMID 36149504›Full record

ArticleArchives of microbiology2022

Analysis of the A549 cell line affected by anticancer bioactive compounds of Actinomycetes isolated from saline soils.

Amin Khoshakhlagh, Saeid Abroun, Seyed Soheil Aghaei, Mohammad Soleimani, Mohammad Reza Zolfaghari

Abstract read
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In one paragraph

Article in Archives of microbiology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.3field-weighted citation impact, top 37% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 3 institutions in 1 country.

Amin KhoshakhlaghDepartment of Microbiology, Qom Branch, Islamic Azad University, Qom, Iran.ORCID http://orcid.org/0000-0002-5528-849X
Saeid AbrounDepartment of Hematology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, 14115-11, Iran. abroun@modares.ac.ir.ORCID http://orcid.org/0000-0001-9459-4994
Seyed Soheil AghaeiDepartment of Microbiology, Qom Branch, Islamic Azad University, Qom, Iran.ORCID http://orcid.org/0000-0003-2144-1860
Mohammad SoleimaniDepartment of Microbiology, Faculty of Medicine, AJA University of Medical Sciences, Tehran, Iran.ORCID http://orcid.org/0000-0002-8270-9499
Mohammad Reza ZolfaghariDepartment of Microbiology, Qom Branch, Islamic Azad University, Qom, Iran.ORCID http://orcid.org/0000-0003-1377-1990
Qom Islamic Azad University · IRAja University of Medical Sciences · IRTarbiat Modares University · IR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Actinomycetes are filamentous bacteria and the residents of the soil, prone to produce bioactive metabolites. This research aimed to isolate, classify, and investigate the anticancer properties of Actinomycetes secondary metabolites from various saline soils of Qom province. Actinomycetes isolates were molecularly recognized by 16SrRNA gene sequencing after the PCR procedure. The A549 cell line was then exposed to bacterial metabolites to find their cytotoxicity by MTT assay and their capacity to cause apoptosis by Flow cytometry. The expression levels of the bax and bcl-2 genes were determined using Real-time PCR. Bacterial metabolites were distinct by HPLC and GC-MS assays. Sequencing identified three novel Actinomycetes strains, Streptomyces griseoflavus, Streptomyces calvus, and Kitasatospora phosalacineus. The IC

Indexed as

ActinobacteriaAntineoplastic AgentsA549 CellsActinomycesbcl-2-Associated X ProteinHumansSoilAntineoplastic Agentsbcl-2-Associated X ProteinSoilA549ActinomycetesAnticancerFlow cytometryReal-time PCRSalin Soils

Identifiers

PMID36149504
OpenAlexW4296793675

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.