ArticlePlant physiology2022
Arabidopsis plastid carbonic anhydrase βCA5 is important for normal plant growth.
Article in Plant physiology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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16 citing papers in PubMed, 26 citations in OpenAlex.
- From fundamental understanding to engineering of carboxysomes for biotechnological applications.Plant communications · 2026Review
- Parsing the stepwise molecular evolution of C4-associated β-carbonic anhydrase in the genus Flaveria.Plant physiology · 2026Article
- Genetic identification and characterization of novel loci for flag leaf morphology traits in Chinese endemic wheat.The plant genome · 2026Article
- Mitochondrial β-carbonic anhydrase is a conserved metabolic rheostat for branched-chain amino acid catabolism and metabolic flexibility.The New phytologist · 2026Article
- Absence of plastidic carbonic anhydrases affects plant growth but not photosynthesis.Plant physiology · 2026Article
- Intermediate evolutionary state of motile sperm and pollen tubes in the extant gymnospermProceedings of the National Academy of Sciences of the United States of America · 2026Article
- Understanding carboxysomes to enhance carbon fixation in crops.Biochemical Society transactions · 2025Review
- Effect of the Absence of α Carbonic Anhydrase 2 on the PSII Light-Harvesting Complex Size inPlants (Basel, Switzerland) · 2025Article
- Photosynthetic Electron Flows and Networks of Metabolite Trafficking to Sustain Metabolism in Photosynthetic Systems.Plants (Basel, Switzerland) · 2024Review
- Engineering the cyanobacterial ATP-driven BCT1 bicarbonate transporter for functional targeting to C3 plant chloroplasts.Journal of experimental botany · 2024Article
- Carbonic anhydrases in the cell wall and plasma membrane ofFrontiers in molecular biosciences · 2024Article
- Rewiring of primary metabolism for ammonium recycling under short-term low COFrontiers in plant science · 2024Article
- One step further toward a crop CO2-concentrating mechanism.Journal of experimental botany · 2023Article
- LCIB functions as a carbonic anhydrase: evidence from yeast and Arabidopsis carbonic anhydrase knockout mutants.Photosynthesis research · 2023Article
- OsαCA1 Affects Photosynthesis, Yield Potential, and Water Use Efficiency in Rice.International journal of molecular sciences · 2023Article
- Small Structural Differences Govern the Carbonic Anhydrase II Inhibition Activity of Cytotoxic Triterpene Acetazolamide Conjugates.Molecules (Basel, Switzerland) · 2023Article
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Carbonic anhydrases (CAs) are zinc-metalloenzymes that catalyze the interconversion of CO2 and HCO3-. In heterotrophic organisms, CAs provide HCO3- for metabolic pathways requiring a carboxylation step. Arabidopsis (Arabidopsis thaliana) has 14 α- and β-type CAs, two of which are plastid CAs designated as βCA1 and βCA5. To study their physiological properties, we obtained knock-out (KO) lines for βCA1 (SALK_106570) and βCA5 (SALK_121932). These mutant lines were confirmed by genomic PCR, RT-PCR, and immunoblotting. While βca1 KO plants grew normally, growth of βca5 KO plants was stunted under ambient CO2 conditions of 400 µL L-1; high CO2 conditions (30,000 µL L-1) partially rescued their growth. These results were surprising, as βCA1 is more abundant than βCA5 in leaves. However, tissue expression patterns of these genes indicated that βCA1 is expressed only in shoot tissue, while βCA5 is expressed throughout the plant. We hypothesize that βCA5 compensates for loss of βCA1 but, owing to its expression being limited to leaves, βCA1 cannot compensate for loss of βCA5. We also demonstrate that βCA5 supplies HCO3- required for anaplerotic pathways that take place in plastids, such as fatty acid biosynthesis.
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