Evidence map›Paper›PMID 36145817›Full record

ArticlePlants (Basel, Switzerland)2022

Comparative Transcriptome Analysis of Two Sugarcane Cultivars in Response to Paclobutrazol Treatment.

Ronghua Zhang, Haibi Li, Yiyun Gui, Jinju Wei, Kai Zhu, Hui Zhou, Prakash Lakshmanan, Lianying Mao, Manman Lu, Junxian Liu and 3 more

Open access · goldAbstract read
In one paragraph

Article in Plants (Basel, Switzerland), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.2field-weighted citation impact, top 33% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 2 institutions in 1 country.

Ronghua ZhangSugarcane Research Center, Chinese Academy of Agricultural Sciences, Nanning 530007, China.
Haibi LiGuangxi South Subtropical Agricultural Science Research Institute, Guangxi Academy of Agricultural Sciences, Longzhou 532415, China.ORCID 0000-0002-3418-7374
Yiyun GuiSugarcane Research Center, Chinese Academy of Agricultural Sciences, Nanning 530007, China.
Jinju WeiSugarcane Research Center, Chinese Academy of Agricultural Sciences, Nanning 530007, China.
Kai ZhuSugarcane Research Center, Chinese Academy of Agricultural Sciences, Nanning 530007, China.
Hui ZhouSugarcane Research Center, Chinese Academy of Agricultural Sciences, Nanning 530007, China.
Prakash LakshmananSugarcane Research Center, Chinese Academy of Agricultural Sciences, Nanning 530007, China.ORCID 0000-0003-1725-0391
Lianying MaoSugarcane Research Center, Chinese Academy of Agricultural Sciences, Nanning 530007, China.
Manman LuSugarcane Research Center, Chinese Academy of Agricultural Sciences, Nanning 530007, China.
Junxian LiuSugarcane Research Center, Chinese Academy of Agricultural Sciences, Nanning 530007, China.
Youxiong QueNational Engineering Research Center for Sugarcane, Fujian Agriculture and Forestry University, Fuzhou 350002, China.ORCID 0000-0003-1111-5834
Song LiSugarcane Research Center, Chinese Academy of Agricultural Sciences, Nanning 530007, China.
Xihui LiuSugarcane Research Center, Chinese Academy of Agricultural Sciences, Nanning 530007, China.
Guangxi Academy of Agricultural Science · CNFujian Agriculture and Forestry University · CN

Funding

Agricultural Sciences Foundation of Guangxi Academy GNK2021YT005Agricultural Sciences Foundation of Guangxi Academy GNK2021YT014National Natural Science Foundation of China 32101696National Natural Science Foundation of China 32160486Regional Foundation of Guangxi GKZY20198005
6 · The paper itself

Abstract

Sugarcane is an important crop across the globe, and the rapid multiplication of excellent cultivars is an important object of the sugarcane industry. As one of the plant growth regulators, paclobutrazol (PBZ) has been frequently used in the tissue culture of sugarcane seedlings. However, little is known about the molecular mechanisms of response to PBZ in this crop. Here, we performed a comparative transcriptome analysis between sensitive (LC05-136) and non-sensitive (GGZ001) sugarcane cultivars treated by PBZ at three time points (0 d, 10 d, and 30 d) using RNA sequencing (RNA-Seq). The results showed that approximately 70.36 Mb of clean data for each sample were generated and assembled into 239,212 unigenes. A total of 6108 and 4404 differentially expressed genes (DEGs) were identified within the sensitive and non-sensitive sugarcane cultivars, respectively. Among them, DEGs in LC05-136 were most significantly enriched in the photosynthesis and valine, leucine and isoleucine degradation pathways, while in GGZ001, DEGs associated with ion channels and plant-pathogen interaction were mainly observed. Notably, many interesting genes, including those encoding putative regulators, key components of photosynthesis, amino acids degradation and glutamatergic synapse, were identified, revealing their importance in the response of sugarcane to PBZ. Furthermore, the expressions of sixteen selected DEGs were tested by quantitative reverse transcription PCR (RT-qPCR), confirming the reliability of the RNA-seq data used in this study. These results provide valuable information regarding the transcriptome changes in sugarcane treated by PBZ and provide an insight into understanding the molecular mechanisms underlying the resistance to PBZ in sugarcane.

Indexed as

comparative transcriptome analysispaclobutrazolRNA−seqsugarcane

Identifiers

PMID36145817
PMCPMC9502373
OpenAlexW4296350107

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.