ArticleNanomaterials (Basel, Switzerland)2022
Number Concentration Measurements of Polystyrene Submicrometer Particles.
Article in Nanomaterials (Basel, Switzerland), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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7 citing papers in PubMed.
- Liter-Scale Precision Synthesis and Measurement of Nano-to-Micrometer Scale PSNPs with Graphene Liquid Cells.ACS omega · 2026Article
- Heat-Inactivated Selenium Nanoparticle-EnrichedMicroorganisms · 2026Article
- Intermethod Characterization of Commercially Available Extracellular Vesicles as Reference Materials.Biomolecules · 2025Article
- Comparative Analysis of Sample Loop and Counting Bead-Based Methods for Size-Dependent Bias in Flow Cytometry.Analytical chemistry · 2025Article
- Derivation of Particle Number Concentration from the Size Distribution: Theory and Applications.Analytical chemistry · 2025Article
- Article
- Use of a sample injection loop for an accurate measurement of particle number concentration by flow cytometry.Analytical and bioanalytical chemistry · 2024Article
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Authors and funding
12 authors.
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Abstract
The number of techniques to measure number concentrations and size distributions of submicrometer particles has recently increased. Submicrometer particle standards are needed to improve the accuracy and reproducibility of these techniques. The number concentrations of fluorescently labeled polystyrene submicrometer sphere suspensions with nominal 100 nm, 200 nm and 500 nm diameters were measured using seven different techniques. Diameter values were also measured where possible. The diameter values were found to agree within 20%, but the number concentration values differed by as much as a factor of two. Accuracy and reproducibility related with the different techniques are discussed with the goal of using number concentration standards for instrument calibration. Three of the techniques were used to determine SI-traceable number concentration values, and the three independent values were averaged to give consensus values. This consensus approach is proposed as a protocol for certifying SI-traceable number concentration standards.
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