Evidence map›Paper›PMID 36144829›Full record

ArticleMolecules (Basel, Switzerland)2022

A Label-Free Fluorescence Aptasensor Based on G-Quadruplex/Thioflavin T Complex for the Detection of Trypsin.

Pan Gu, Yangfan Lu, Shanni Li, Changbei Ma

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecules (Basel, Switzerland), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.6field-weighted citation impact, top 35% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 7 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Pan GuSchool of Life Sciences, Central South University, Changsha 410013, China.
Yangfan LuSchool of Life Sciences, Central South University, Changsha 410013, China.ORCID 0000-0001-8072-775X
Shanni LiSchool of Life Sciences, Central South University, Changsha 410013, China.
Changbei MaSchool of Life Sciences, Central South University, Changsha 410013, China.
Central South University · CN

Funding

National Natural Science Foundation of China 21205142
6 · The paper itself

Abstract

A novel, label-free fluorescent assay has been developed for the detection of trypsin by using thioflavin T as a fluorescent probe. A specific DNA aptamer can be combined by adding cytochrome c. Trypsin hydrolyzes the cytochrome c into small peptide fragments, exposing the G-quadruplex part of DNA aptamer, which has a high affinity for thioflavin T, which then enhances the fluorescence intensity. In the absence of trypsin, the fluorescence intensity was inhibited as the combination of cytochrome c and the DNA aptamer impeded thioflavin T's binding. Thus, the fluorescent biosensor showed a linear relationship from 0.2 to 60 μg/mL with a detection limit of 0.2 μg/mL. Furthermore, the proposed method was also successfully employed for determining trypsin in biological samples. This method is simple, rapid, cheap, and selective and possesses great potential for the detection of trypsin in bioanalytical and biological samples and medical diagnoses.

Indexed as

Aptamers, NucleotideBiosensing TechniquesG-QuadruplexesBenzothiazolesCytochromes cFluorescenceFluorescent DyesLimit of DetectionPeptide FragmentsSpectrometry, FluorescenceTrypsinAptamers, NucleotideBenzothiazolesCytochromes cFluorescent DyesPeptide Fragmentsthioflavin TTrypsincytochrome cDNA aptamerlabel-freethioflavin Ttrypsin

Identifiers

PMID36144829
PMCPMC9503660
OpenAlexW4296456113

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.