ArticleMicroorganisms2022
Effect of Calcium Ion Supplementation on Oral Microbial Composition and Biofilm Formation In Vitro.
Article in Microorganisms, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
10 citing papers in PubMed, 17 citations in OpenAlex.
- Dental implant nanotopography for peri-implant tissue stability: soft-tissue sealing, innate immune calibration, and active biointerface regulation.International journal of implant dentistry · 2026Review
- Concentrations of selected human milk components influence the infant oral microbiome to a greater degree than estimated intakes.Frontiers in cellular and infection microbiology · 2026Article
- Biosorption-based elimination of hexavalent chromium using Bacillus cereus F4810/72 isolated from tannery effluents.Biodegradation · 2025Article
- The metabolic pathway of THF-degrading composite bacteria and its immobilized microspheres.Antonie van Leeuwenhoek · 2025Article
- The effect of dental material type and masticatory forces on periodontitis-derived subgingival microbiomes.Biofilm · 2024Article
- A preliminary study on calcifying nanoparticles in dental plaque: Isolation, characterization, and potential mineralization mechanism.Clinical and experimental dental research · 2024Article
- Characterization of MdpS: an in-depth analysis of a MUC5B-degrading protease fromFrontiers in microbiology · 2024Article
- Article
- Perspective: The viscoelastic properties of biofilm infections and mechanical interactions with phagocytic immune cells.Frontiers in cellular and infection microbiology · 2023Review
- Oral Microorganisms and Biofilms: New Insights to Defeat the Main Etiologic Factor of Oral Diseases.Microorganisms · 2022Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
Abstract
The oral cavity contains a variety of ecological niches with very different environmental conditions that shape biofilm structure and composition. The space between the periodontal tissue and the tooth surface supports a unique anaerobic microenvironment that is bathed in the nutrient-rich gingival crevicular fluid (GCF). During the development of periodontitis, this environment changes and clinical findings reported a sustained level of calcium ion concentration in the GCF collected from the periodontal pockets of periodontitis patients. Here, we report the effect of calcium ion supplementation on human oral microbial biofilm formation and community composition employing an established SHI medium-based in vitro model system. Saliva-derived human microbial biofilms cultured in calcium-supplemented SHI medium (SHICa) exhibited a significant dose-dependent increase in biomass and metabolic activity. The effect of SHICa medium on the microbial community composition was evaluated by 16S rRNA gene sequencing using saliva-derived microbial biofilms from healthy donors and periodontitis subjects. In this study, intracellular microbial genomic DNA (iDNA) and extracellular DNA (eDNA) were analyzed separately at the genus level. Calcium supplementation of SHI medium had a differential impact on iDNA and eDNA in the biofilms derived from healthy individuals compared to those from periodontitis subjects. In particular, the genus-level composition of the eDNA portion was distinct between the different biofilms. This study demonstrated the effect of calcium in a unique microenvironment on oral microbial complex supporting the dynamic transformation and biofilm formation.
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