Evidence map›Paper›PMID 36144364›Full record

ArticleMicroorganisms2022

Development of a Molecular Marker Based on the Mitochondrial Genome for Detection of

Mauricio Durigan, Emma Patregnani, Gopal R Gopinath, Laura Ewing-Peeples, Chaeyoon Lee, Helen R Murphy, Sonia Almeria, Hediye Nese Cinar, Flavia Negrete, Alexandre J da Silva

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Microorganisms, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
1.4field-weighted citation impact, top 22% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 8 citations in OpenAlex.

  1. SenescentMicroorganisms · 2026
    Article
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  3. Article
  4. Article
  5. Microorganisms · 2023
    Article
  6. Microorganisms · 2023
    Article
  7. Novel insights on the genetic population structure of human-infectingCurrent research in parasitology & vector-borne diseases · 2023
    Article
  8. Hastening Progress inMicroorganisms · 2022
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 1 institution in 1 country.

Mauricio DuriganOffice of Applied Research and Safety Assessment, Center for Food Safety and Applied Nutrition (CFSAN), U.S. Food and Drug Administration, Laurel, MD 20708, USA.
Emma PatregnaniOffice of Applied Research and Safety Assessment, Center for Food Safety and Applied Nutrition (CFSAN), U.S. Food and Drug Administration, Laurel, MD 20708, USA.
Gopal R GopinathOffice of Applied Research and Safety Assessment, Center for Food Safety and Applied Nutrition (CFSAN), U.S. Food and Drug Administration, Laurel, MD 20708, USA.ORCID 0000-0003-2577-7548
Laura Ewing-PeeplesOffice of Applied Research and Safety Assessment, Center for Food Safety and Applied Nutrition (CFSAN), U.S. Food and Drug Administration, Laurel, MD 20708, USA.
Chaeyoon LeeOffice of Applied Research and Safety Assessment, Center for Food Safety and Applied Nutrition (CFSAN), U.S. Food and Drug Administration, Laurel, MD 20708, USA.
Helen R MurphyOffice of Applied Research and Safety Assessment, Center for Food Safety and Applied Nutrition (CFSAN), U.S. Food and Drug Administration, Laurel, MD 20708, USA.
Sonia AlmeriaOffice of Applied Research and Safety Assessment, Center for Food Safety and Applied Nutrition (CFSAN), U.S. Food and Drug Administration, Laurel, MD 20708, USA.ORCID 0000-0002-0558-5488
Hediye Nese CinarOffice of Applied Research and Safety Assessment, Center for Food Safety and Applied Nutrition (CFSAN), U.S. Food and Drug Administration, Laurel, MD 20708, USA.
Flavia NegreteOffice of Applied Research and Safety Assessment, Center for Food Safety and Applied Nutrition (CFSAN), U.S. Food and Drug Administration, Laurel, MD 20708, USA.
Alexandre J da SilvaOffice of Applied Research and Safety Assessment, Center for Food Safety and Applied Nutrition (CFSAN), U.S. Food and Drug Administration, Laurel, MD 20708, USA.
Center for Food Safety and Applied Nutrition · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Cyclospora cayetanensis is a coccidian parasite that causes diarrheal illness outbreaks worldwide. The development of new laboratory methods for detection of C. cayetanensis is of critical importance because of the high potential for environmental samples to be contaminated with a myriad of microorganisms, adversely impacting the specificity when testing samples from various sources using a single molecular assay. In this study, a new sequencing-based method was designed targeting a specific fragment of C. cayetanensis cytochrome oxidase gene and developed as a complementary method to the TaqMan qPCR present in the U.S. FDA BAM Chapter 19b and Chapter 19c. The comparative results between the new PCR protocol and the qPCR for detection of C. cayetanensis in food and water samples provided similar results in both matrices with the same seeding level. The target region and primers in the protocol discussed in this study contain sufficient Cyclospora-specific sequence fidelity as observed by sequence comparison with other Eimeriidae species. The sequence of the PCR product appears to represent a robust target for identifying C. cayetanensis on samples from different sources. Such a sensitive method for detection of C. cayetanensis would add to the target repertoire of qPCR-based screening strategies for food and water samples.

Indexed as

agricultural waterCyclospora cayetanensisdetection markerenvironmental samplesfood safety

Identifiers

PMID36144364
PMCPMC9504131
OpenAlexW4294162997

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.