Evidence map›Paper›PMID 36144139›Full record

ArticleMicromachines2022

Rapid and Continuous Cryopreservation of Stem Cells with a 3D Micromixer.

Lin Ding, Sajad Razavi Bazaz, Jesus Shrestha, Hoseyn A Amiri, Sima Mas-Hafi, Balarka Banerjee, Graham Vesey, Morteza Miansari, Majid Ebrahimi Warkiani

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Micromachines, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Review
  3. Review
  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Lin DingSchool of Biomedical Engineering, University of Technology Sydney, Sydney, NSW 2007, Australia.
Sajad Razavi BazazSchool of Biomedical Engineering, University of Technology Sydney, Sydney, NSW 2007, Australia.ORCID 0000-0002-6419-3361
Jesus ShresthaSchool of Biomedical Engineering, University of Technology Sydney, Sydney, NSW 2007, Australia.ORCID 0000-0003-3692-6676
Hoseyn A AmiriMicro+Nanosystems & Applied Biophysics Laboratory, Department of Mechanical Engineering, Babol Noshirvani University of Technology, P.O. Box 484, Babol 47148-71167, Iran.ORCID 0000-0001-5608-4248
Sima Mas-HafiMicro+Nanosystems & Applied Biophysics Laboratory, Department of Mechanical Engineering, Babol Noshirvani University of Technology, P.O. Box 484, Babol 47148-71167, Iran.ORCID 0000-0002-0058-2659
Balarka BanerjeeRegeneus Ltd., Paddington, Sydney, NSW 2021, Australia.
Graham VeseyRegeneus Ltd., Paddington, Sydney, NSW 2021, Australia.
Morteza MiansariMicro+Nanosystems & Applied Biophysics Laboratory, Department of Mechanical Engineering, Babol Noshirvani University of Technology, P.O. Box 484, Babol 47148-71167, Iran.ORCID 0000-0002-3290-0500
Majid Ebrahimi WarkianiSchool of Biomedical Engineering, University of Technology Sydney, Sydney, NSW 2007, Australia.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Cryopreservation is the final step of stem cell production before the cryostorage of the product. Conventional methods of adding cryoprotecting agents (CPA) into the cells can be manual or automated with robotic arms. However, challenging issues with these methods at industrial-scale production are the insufficient mixing of cells and CPA, leading to damage of cells, discontinuous feeding, the batch-to-batch difference in products, and, occasionally, cross-contamination. Therefore, the current study proposes an alternative way to overcome the abovementioned challenges; a highly efficient micromixer for low-cost, continuous, labour-free, and automated mixing of stem cells with CPA solutions. Our results show that our micromixer provides a more homogenous mixing of cells and CPA compared to the manual mixing method, while the cell properties, including surface markers, differentiation potential, proliferation, morphology, and therapeutic potential, are well preserved.

Indexed as

3D printingbioprocessing industrycryopreservationmicrofluidicsstem cells

Identifiers

PMID36144139
PMCPMC9500807

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.