Evidence map›Paper›PMID 36144017›Full record

ArticleMicromachines2022

Nucleic Acid Detection with Ion Concentration Polarization Microfluidic Chip for Reduced Cycle Numbers of Polymerase Chain Reaction.

Chengzhuang Yu, Shijie Dai, Shanshan Li, Junwei Li, Hezhi Hu, Jiyu Meng, Chunyang Wei, Jie Jayne Wu

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Micromachines, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.2field-weighted citation impact, top 53% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 3 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 2 countries.

Chengzhuang YuHebei Key Laboratory of Smart Sensing and Human-Robot Interactions, School of Mechanical Engineering, Hebei University of Technology, Tianjin 300130, China.
Shijie DaiHebei Key Laboratory of Smart Sensing and Human-Robot Interactions, School of Mechanical Engineering, Hebei University of Technology, Tianjin 300130, China.
Shanshan LiHebei Key Laboratory of Smart Sensing and Human-Robot Interactions, School of Mechanical Engineering, Hebei University of Technology, Tianjin 300130, China.ORCID 0000-0001-8293-9676
Junwei LiInstitute of Biophysics, School of Health Sciences and Biomedical Engineering, Hebei University of Technology, Tianjin 300130, China.ORCID 0000-0002-1724-4752
Hezhi HuState Key Laboratory of Reliability and Intelligence of Electrical Equipment, School of Electrical Engineering, Hebei University of Technology, Tianjin 300130, China.
Jiyu MengHebei Key Laboratory of Smart Sensing and Human-Robot Interactions, School of Mechanical Engineering, Hebei University of Technology, Tianjin 300130, China.
Chunyang WeiHebei Key Laboratory of Smart Sensing and Human-Robot Interactions, School of Mechanical Engineering, Hebei University of Technology, Tianjin 300130, China.
Jie Jayne WuDepartment of Electrical Engineering and Computer Science, The University of Tennessee, Knoxville, TN 37996, USA.
Hebei University of Technology · CNUniversity of Tennessee at Knoxville · US

Funding

Department of Human Resources and Social Security of Hebei Province C20200314 and C20210337Fund for Distinguish Young Scholars in Tianjin 2018 3rd Round 180191Hebei Natural Science Foundation E2022202127, E2020202101 and F2021202001Hebei Science and Technology Foundation 19271707DJiangsu Key Laboratory of Advanced Manufacturing Equipment and Technology FMZ202016National Natural Science Foundation of China 51728502
6 · The paper itself

Abstract

Nucleic acid detection is widely used in disease diagnosis, food safety, environmental monitoring and many other research fields. The continuous development of rapid and sensitive new methods to detective nucleic acid is very important for practical application. In this study, we developed a rapid nucleic-acid detection method using polymerase chain reaction (PCR) combined with electrokinetic preconcentration based on ion concentration polarization (ICP). Using a Nafion film, the proposed ICP microfluidic chip is utilized to enrich the nucleic acid molecules amplified by PCR thermal cycles. To demonstrate the capability of the microfluidic device and the hybrid nucleic-acid detection method, we present an animal-derived component detection experiment for meat product identification applications. With the reduced cycle numbers of 24 cycles, the detection can be completed in about 35 min. The experimental results show that this work can provide a microfluidic device and straightforward method for rapid detection of nucleic acids with reduced cycle numbers.

Indexed as

electrokinetic preconcentrationion concentration polarizationmicrofluidicnucleic acid detectionpolymerase chain reaction

Identifiers

PMID36144017
PMCPMC9506297
OpenAlexW4293761083

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.