ArticleInternational journal of molecular sciences2022
Polymicrobial Biofilm Organization of
Article in International journal of molecular sciences, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed.
- L-fucose-dependent biofilm formation byBiofilm · 2026Article
- Serial-Passage Assessment Shows No Confirmed Resistance Development to Norway Spruce (Picea Abies) Resin in Bacterial Species Relevant to Wound Infection.MicrobiologyOpen · 2026Article
- Graphene Oxide and Methylglyoxal: a combined strategy against chronic wound pathogens.Applied microbiology and biotechnology · 2026Article
- MRSA-dominant colonization and synergistic pathogenicity withJournal of orthopaedic translation · 2026Article
- Staphylococcus aureus biofilms: molecular mechanisms, resistance determinants, and emerging therapeutic strategies.Molecular biology reports · 2026Review
- Bacteriophage therapy for antimicrobial-resistant, biofilm‑associated diabetic foot infection: delivery routes, phage antibiotic synergy, and practical wound‑care integration.Archives of microbiology · 2026Review
- Characterization of secondary bacterial infections in Buruli ulcer disease in Ghana: a focus on antimicrobial resistant Staphylococcus aureus.BMC infectious diseases · 2026Article
- A Comprehensive Review of Antimicrobial Peptides and Smart Biomaterials in Chronic Wound Therapy: Overcoming Biofilms, Resistance, and Translational Barriers.International journal of molecular sciences · 2026Review
- L-Fucose-Dependent Biofilm Formation bybioRxiv : the preprint server for biology · 2026Article
- Biofilms in clinical infection: pathophysiology, diagnosis, and the evolving therapeutic landscape.Journal of clinical microbiology · 2026Review
- GlnK Regulates the Type III Secretion System by Modulating NtrB-NtrC Homeostasis inMicroorganisms · 2026Article
- Can commensals alter pathogen's antibiotic resistance during co-culture?Journal of medical microbiology · 2026Article
- Critical Assessment of Intrinsic Antibacterial Properties and Photothermal Therapy Potential of MXene Nanosheets.ACS applied nano materials · 2026Article
- Betulinic Acid Eradicates Implant-Associated Infections by Disrupting the S. aureus Biofilm Matrix and Potentiating Host Immune Clearance.Microbial biotechnology · 2026Article
- Nanocarrier-Integrated Topical Dosage Forms for Biofilm-Infected Wounds: Balancing Wound Retention, Biofilm Matrix Penetration, and Translational Feasibility.International journal of nanomedicine · 2026Review
- Multidimensional Regulatory Mechanisms and Targeted Therapeutic Strategies for Inhibited Keratinocyte Proliferation in Diabetic Wounds.Drug design, development and therapy · 2026Review
- Polyhexamethylene guanidine derived carbon dots with antibacterial and immunoregulatory properties loaded on carboxymethyl chitosan and ε-poly-L-lysine hydrogel to achieve sustained drug release for treatingMaterials today. Bio · 2025Article
- 16S rRNA sequencing-based analysis of sputum microbiome in patients with acute exacerbations of chronic obstructive pulmonary disease: retrospective cohort study.Journal of thoracic disease · 2025Article
- Polymicrobial Biofilms: Interkingdom Interactions, Resistance and Therapeutic Strategies.Microbial biotechnology · 2025Review
- Strain-specific interspecies interactions between co-isolated pairs of Staphylococcus aureus and Helcococcus kunzii from chronic wounds.BMC microbiology · 2025Article
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Authors and funding
6 authors.
Funding
Abstract
Biofilm on the skin surface of chronic wounds is an important step that involves difficulties in wound healing. The polymicrobial nature inside this pathogenic biofilm is key to understanding the chronicity of the lesion. Few in vitro models have been developed to study bacterial interactions inside this chronic wound. We evaluated the biofilm formation and the evolution of bacteria released from this biofilm on the two main bacteria isolated in this condition, Staphylococcus aureus and Pseudomonas aeruginosa, using a dynamic system (BioFlux™ 200) and a chronic wound-like medium (CWM) that mimics the chronic wound environment. We observed that all species constituted a faster biofilm in the CWM compared to a traditional culture medium (p < 0.01). The percentages of biofilm formation were significantly higher in the mixed biofilm compared to those determined for the bacterial species alone (p < 0.01). Biofilm organization was a non-random structure where S. aureus aggregates were located close to the wound surface, whereas P. aeruginosa was located deeper in the wound bed. Planktonic biofilm-detached bacteria showed decreased growth, overexpression of genes encoding biofilm formation, and an increase in the mature biofilm biomass formed. Our data confirmed the impact of the chronic wound environment on biofilm formation and on bacterial lifecycle inside the biofilm.
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