Evidence map›Paper›PMID 36142320›Full record

ArticleInternational journal of molecular sciences2022

Fluorescence Spectroscopy of Low-Level Endogenous β-Adrenergic Receptor Expression at the Plasma Membrane of Differentiating Human iPSC-Derived Cardiomyocytes.

Philipp Gmach, Marc Bathe-Peters, Narasimha Telugu, Duncan C Miller, Paolo Annibale

Abstract read
In one paragraph

Article in International journal of molecular sciences, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Special Issue "GPCR: Roles in Cell Development and Disease".International journal of molecular sciences · 2023
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Philipp GmachMax Delbrück Center for Molecular Medicine in the Helmholtz Association, 13125 Berlin, Germany.ORCID 0000-0002-5258-0321
Marc Bathe-PetersMax Delbrück Center for Molecular Medicine in the Helmholtz Association, 13125 Berlin, Germany.
Narasimha TeluguMax Delbrück Center for Molecular Medicine in the Helmholtz Association, 13125 Berlin, Germany.
Duncan C MillerMax Delbrück Center for Molecular Medicine in the Helmholtz Association, 13125 Berlin, Germany.
Paolo AnnibaleMax Delbrück Center for Molecular Medicine in the Helmholtz Association, 13125 Berlin, Germany.

Funding

Deutsche Forschungsgemeinschaft 421152132 SFB 1423 subproject C03Deutsche Forschungsgemeinschaft SFB 1470 subproject A01
6 · The paper itself

Abstract

The potential of human-induced pluripotent stem cells (hiPSCs) to be differentiated into cardiomyocytes (CMs) mimicking adult CMs functional morphology, marker genes and signaling characteristics has been investigated since over a decade. The evolution of the membrane localization of CM-specific G protein-coupled receptors throughout differentiation has received, however, only limited attention to date. We employ here advanced fluorescent spectroscopy, namely linescan Fluorescence Correlation Spectroscopy (FCS), to observe how the plasma membrane abundance of the β

Indexed as

Induced Pluripotent Stem CellsAdultCell DifferentiationCell MembraneCells, CulturedHumansMyocytes, CardiacReceptors, Adrenergic, betaRNA, MessengerSpectrometry, FluorescenceReceptors, Adrenergic, betaRNA, MessengercardiomyocyteCRISPR/Cas9differentiationFCSfluorescencefluorescence correlation spectroscopyGPCRhiPSC-CMβ-adrenergic receptors

Identifiers

PMID36142320
PMCPMC9499492

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.