Analysis of Phosphatase Activity in a Droplet-Based Microfluidic Chip.

Bala Murali Krishna Vasamsetti, Yeon-Jun Kim, Jung Hoon Kang, Jae-Won Choi

Open access · goldAbstract read

In one paragraph

Article in Biosensors, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

0.2field-weighted citation impact, top 57% of its field

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 2 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Bala Murali Krishna VasamsettiDepartment of Biomedical Science, Cheongju University, Cheongju 28160, Korea.ORCID 0000-0001-5529-418X

Yeon-Jun KimDepartment of Biomedical Science, Cheongju University, Cheongju 28160, Korea.ORCID 0000-0003-4732-2501

Jung Hoon KangDepartment of Biomedical Science, Cheongju University, Cheongju 28160, Korea.

Jae-Won ChoiDepartment of Biomedical Science, Cheongju University, Cheongju 28160, Korea.ORCID 0000-0001-9533-9581

Cheongju University · KR

Funding

Korea Institute for Advancement of Technology P0017805National Research Foundation of Korea 2021R1F1A1062994National Research Foundation of Korea 2021RIS-001

6 · The paper itself

Abstract

We report analysis of phosphatase activity and inhibition on droplet-based microfluidic chips. Phosphatases are such attractive potential drug targets because abnormal phosphatase activity has been implicated in a variety of diseases including cancer, neurological disorders, diabetes, osteoporosis, and obesity. So far, several methods for assessing phosphatase activity have been reported. However, they require a large sample volume and additional chemical modifications such as fluorescent dye conjugation and nanomaterial conjugation, and are not cost-effective. In this study, we used an artificial phosphatase substrate 3-

Indexed as

Microfluidic Analytical TechniquesMicrofluidicsBiological AssayDual-Specificity PhosphatasesEnzyme InhibitorsFluorescent DyesDual-Specificity PhosphatasesEnzyme InhibitorsFluorescent Dyesdroplet-based microfluidicsDUSP22ethyl-3,4-dephostatinmicrodropletphosphatase activityPTP assay

Identifiers

PMID36140125

DOI10.3390/bios12090740

PMCPMC9496282

OpenAlexW4296087223

What OpenQuestion holds

Textmetadata

LicenceCC BY

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.