Evidence map›Paper›PMID 36140072›Full record

ArticleBiosensors2022

Functional Characterization and Phenotyping of Protoplasts on a Microfluidics-Based Flow Cytometry.

Xingda Dai, Shuaihua Zhang, Siyuan Liu, Hang Qi, Xuexin Duan, Ziyu Han, Jiehua Wang

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biosensors, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
1.3field-weighted citation impact, top 18% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 9 citations in OpenAlex.

  1. Review
  2. Review
  3. Review
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Xingda DaiSchool of Environmental Science and Engineering, Tianjin University, Weijin Rd. 92, Tianjin 300072, China.
Shuaihua ZhangState Key Laboratory of Precision Measuring Technology and Instruments, College of Precision Instruments and Optoelectronics Engineering, Tianjin University, Weijin Rd. 92, Tianjin 300072, China.
Siyuan LiuState Key Laboratory of Precision Measuring Technology and Instruments, College of Precision Instruments and Optoelectronics Engineering, Tianjin University, Weijin Rd. 92, Tianjin 300072, China.
Hang QiState Key Laboratory of Precision Measuring Technology and Instruments, College of Precision Instruments and Optoelectronics Engineering, Tianjin University, Weijin Rd. 92, Tianjin 300072, China.
Xuexin DuanState Key Laboratory of Precision Measuring Technology and Instruments, College of Precision Instruments and Optoelectronics Engineering, Tianjin University, Weijin Rd. 92, Tianjin 300072, China.ORCID 0000-0002-7550-3951
Ziyu HanState Key Laboratory of Precision Measuring Technology and Instruments, College of Precision Instruments and Optoelectronics Engineering, Tianjin University, Weijin Rd. 92, Tianjin 300072, China.
Jiehua WangSchool of Environmental Science and Engineering, Tianjin University, Weijin Rd. 92, Tianjin 300072, China.ORCID 0000-0002-8559-4797
Tianjin University · CN

Funding

National Natural Science Foundation of China 32170413
6 · The paper itself

Abstract

A better understanding of the phenotypic heterogeneity of protoplasts requires a comprehensive analysis of the morphological and metabolic characteristics of many individual cells. In this study, we developed a microfluidic flow cytometry with fluorescence sensor for functional characterization and phenotyping of protoplasts to allow an unbiased assessment of the influence of environmental factors at the single cell level. First, based on the measurement of intracellular homeostasis of reactive oxygen species (ROS) with a DCFH-DA dye, the effects of various external stress factors such as H

Indexed as

ArabidopsisProtoplastsAnthocyaninsCadmiumFlow CytometryHydrogen PeroxideIndoleacetic AcidsMicrofluidicsReactive Oxygen SpeciesAnthocyaninsCadmiumHydrogen PeroxideIndoleacetic AcidsReactive Oxygen Speciesanthocyanidinfluorescent reportermicrofluidic flow cytometryprimary cell wallprotoplastsUV lights

Identifiers

PMID36140072
PMCPMC9496511
OpenAlexW4293413286

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.