Evidence map›Paper›PMID 36138538›Full record

ArticleMetallomics : integrated biometal science2022

MTM1 displays a new function in the regulation of nickel resistance in Saccharomyces cerevisiae.

Naifeng Xu, Yuan Xu, Nathan Smith, Huizhu Chen, Ziguo Guo, Jaekwon Lee, Xiaobin Wu

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Metallomics : integrated biometal science, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.5field-weighted citation impact, top 41% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 5 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 2 countries.

Naifeng XuDevelopment Center of Plant Germplasm Resources, College of Life Sciences, Shanghai Normal University, Shanghai 200234, China.ORCID 0000-0001-6457-7659
Yuan XuDevelopment Center of Plant Germplasm Resources, College of Life Sciences, Shanghai Normal University, Shanghai 200234, China.
Nathan SmithDepartment of Biochemistry and Redox Biology Center, University of Nebraska-Lincoln, Lincoln 68588-0664, Nebraska.
Huizhu ChenDevelopment Center of Plant Germplasm Resources, College of Life Sciences, Shanghai Normal University, Shanghai 200234, China.
Ziguo GuoHubei Inspection Center for Quality and Safety of Agricultural Food, Wuhan 430070, China.
Jaekwon LeeDepartment of Biochemistry and Redox Biology Center, University of Nebraska-Lincoln, Lincoln 68588-0664, Nebraska.
Xiaobin WuDevelopment Center of Plant Germplasm Resources, College of Life Sciences, Shanghai Normal University, Shanghai 200234, China.ORCID 0000-0001-5479-8597
Shanghai Normal University · CNUniversity of Nebraska–Lincoln · USHubei Academy of Agricultural Sciences · CN

Funding

Mechanistic insights into homeostatic copper acquistionR01DK079209 · NIDDK · UNIVERSITY OF NEBRASKA LINCOLN · PI LEE, JAEKWON · 2007 to 2014
$1.9M
NIDDK NIH HHS R01 DK079209
6 · The paper itself

Abstract

Nickel (Ni) is an essential yet toxic trace element. Although a cofactor for many metalloenzymes, nickel function and metabolism is not fully explored in eukaryotes. Molecular biology and metallomic methods were utilized to explore the new physiological functions of nickel in Saccharomyces cerevisiae. Here we showed that MTM1 knockout cells displayed much stronger nickel tolerance than wild-type cells and mitochondrial accumulations of Ni and Fe of mtm1Δ cells dramatically decreased compared to wild-type cells when exposed to excess nickel. Superoxide dismutase 2 (Sod2p) activity in mtm1Δ cells was severely attenuated and restored through Ni supplementation in media or total protein. SOD2 mRNA level of mtm1Δ cells was significantly higher than that in the wild-type strain but was decreased by Ni supplementation. MTM1 knockout afforded resistance to excess nickel mediated through reactive oxygen species levels. Meanwhile, additional Ni showed no significant effect on the localization of Mtm1p. Our study reveals the MTM1 gene plays an important role in nickel homeostasis and identifies a novel function of nickel in promoting Sod2p activity in yeast cells.

Indexed as

MetalloproteinsSaccharomyces cerevisiae ProteinsTrace ElementsCarrier ProteinsMitochondrial ProteinsNickelReactive Oxygen SpeciesRNA, MessengerSaccharomyces cerevisiaeSuperoxide DismutaseCarrier ProteinsMetalloproteinsMitochondrial ProteinsMtm1 protein, S cerevisiaeNickelReactive Oxygen SpeciesRNA, MessengerSaccharomyces cerevisiae ProteinsSuperoxide DismutaseTrace ElementshomeostasisMTM1nickelSod2ptoleranceyeast

Identifiers

PMID36138538
PMCPMC9989664
OpenAlexW4296801545

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.