ArticlePloS one2022
Mitochondrial DNA barcoding of mosquito species (Diptera: Culicidae) in Thailand.
Article in PloS one, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 25 papers, 1 of them a synthesis that pooled it.
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Who cites it
25 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Systematic review of Anopheles abundance and meta-analysis of nonhuman primate malaria infection in mosquitoes in Thailand.Parasites & vectors · 2026Pooled it
- Dengue virus at the landscape scale in Cambodia: Linking climate and land use dynamics with Dengue ecology in Southeast Asia.Research square · 2026Article
- A descriptive assessment of mitochondrial COI genetic diversity and dengue and chikungunya RNA detection in Aedes aegypti across eight provinces in Thailand.Parasites & vectors · 2026Article
- Zoonotic malaria in Southeast Asia's changing landscapes: vector complexity, challenges, and future strategies.Parasites & vectors · 2026Review
- Genetic diversity and gut microbiome of Anopheles mosquitoes in Tamil Nadu by using COI DNA barcoding and 16S rRNA metagenomics.Scientific reports · 2026Article
- Genetic Diversity and Phylogenetic Relationships of Mosquitoes (Diptera: Culicidae) in Central Nepal.Ecology and evolution · 2026Article
- Geometric morphometric analysis of wing size and shape variation inJournal of advanced veterinary and animal research · 2026Article
- An Integrative Assessment ofJournal of tropical medicine · 2026Article
- Article
- Real-time dynamic monitoring and multiplex PCR identification of vector mosquitoes in Zhejiang, China.PLoS neglected tropical diseases · 2025Article
- Genetic diversity and population structure of Aedes caspius in Iran.Scientific reports · 2025Article
- Correction: Mitochondrial DNA barcoding of mosquito species (Diptera: Culicidae) in Thailand.PloS one · 2025Article
- Accurate identification of medically importantCurrent research in parasitology & vector-borne diseases · 2025Article
- Species classification ofCurrent research in parasitology & vector-borne diseases · 2025Article
- DNA Barcoding for the Identification of Adult Mosquitoes (Diptera: Culicidae) in Western Australia.Ecology and evolution · 2024Article
- Barcoding of Italian mosquitoes (BITMO): generation and validation of DNA barcoding reference libraries for native and alien species of Culicidae.Parasites & vectors · 2024Article
- Determination of Species Identity and Genetic Diversity of Aedes aegypti and Other Medically Important Mosquitoes of India Using DNA Barcoding.The American journal of tropical medicine and hygiene · 2024Article
- Integrating wing morphometrics and mitochondrial DNA analysis to assess the filaria vector Mansonia uniformis (Diptera: Culicidae) populations in Thailand.Parasitology research · 2024Article
- Island mosquitoes of Thailand: an update on species diversity and DNA barcoding.Parasitology research · 2024Article
- Insights into the mitochondrial cytochrome oxidase I (mt-COI) gene and wing morphometrics of Anopheles baimaii (Diptera: Culicidae) in malaria-endemic islands of Thailand.Parasitology research · 2024Article
Corrections and comments
- Erratum issued
Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The correct identification of mosquito species is important for effective mosquito vector control. However, the standard morphological identification of mosquito species based on the available keys is not easy with specimens in the field due to missing or damaged morphological features during mosquito collections, often leading to the misidentification of morphologically indistinguishable. To resolve this problem, we collected mosquito species across Thailand to gather genetic information, and evaluated the DNA barcoding efficacy for mosquito species identification in Thailand. A total of 310 mosquito samples, representing 73 mosquito species, were amplified using mitochondrial cytochrome c oxidase subunit I (COI) primers. The average maximum intraspecific genetic variation of the 73 mosquito species was 1% ranged from 0-5.7%. While, average minimum interspecific genetic variation (the distance to the nearest neighbour) of the 73 mosquito species was 7% ranged from 0.3-12.9%. The identification of success rates based on the "Best Match," "Best Close Match," and "All Species Barcodes" methods were 97.7%, 91.6%, and 81%, respectively. Phylogenetic analyses of Anopheles COI sequences demonstrated a clear separation between almost all species (except for those between An. baimaii and An. dirus), with high bootstrap support values (97%-99%). Furthermore, phylogenetic analyses revealed potential sibling species of An. annularis, An. tessellatus, and An. subpictus in Thailand. Our results indicated that DNA barcoding is an effective molecular approach for the accurate identification of mosquitoes in Thailand.
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