ArticleBiology of reproduction2023
Recapitulating folliculogenesis and oogenesis outside the body: encapsulated in vitro follicle growth†.
Article in Biology of reproduction, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
23 citing papers in PubMed, 23 citations in OpenAlex.
- Engineering ovarian asymmetric mechanical niches in a dual-layer microcavity array enhances mouse secondary follicle development and oocyte competence.Bioactive materials · 2027Article
- Phototoxicity of brightfield live-cell imaging on murine ovarian follicles.Archives of gynecology and obstetrics · 2026Article
- Modulating IL-11-dependent matrix stiffness to delay ovarian aging.Nature aging · 2026Article
- In vitro mouse preantral follicle development in 2D and suspension culture: α-MEM vs SAGE 1-step.Reproduction & fertility · 2026Article
- NFYA regulates two sequential genome-wide transcriptional activation events during oocyte to embryo transition.bioRxiv : the preprint server for biology · 2026Article
- Intercellular cyclic nucleotide dynamics mediate oocyte meiosis in mammalian preovulatory follicles.Current topics in developmental biology · 2026Review
- Fertility preservation and subsequent reproductive outcomes in female pediatric patients with hematologic malignancies.Frontiers in endocrinology · 2026Review
- Microscale pressure measurements and optical coherence tomography reveal time-dependent biomechanical stages of ovulation in mice.iScience · 2025Article
- OoCount: a machine-learning-based approach to mouse ovarian follicle counting and classification†.Biology of reproduction · 2025Article
- Production and Use of Customizable Agarose Molds for Scaffold-Free Mouse Ovarian Follicle Culture.Journal of visualized experiments : JoVE · 2025Article
- Primary human ovarian interstitial cells contribute to murine follicle growth through follicle-interstitial paracrine crosstalk.Research square · 2025Article
- Transcriptome Sequencing and Differential Analysis of Ovaries Across Diverse States (Follicular and Non-Follicular Phases).Animals : an open access journal from MDPI · 2025Article
- A spotlight on factors influencing the in vitro folliculogenesis of isolated preantral follicles.Journal of assisted reproduction and genetics · 2024Article
- Follicle-intrinsic and spatially distinct molecular programs drive follicle rupture and luteinization during ex vivo mammalian ovulation.Communications biology · 2024Article
- Novel imaging and biophysical approaches to study tissue hydraulics in mammalian folliculogenesis.Biophysical reviews · 2024Review
- Heterogeneity of ovarian matrisome hydrogels elucidates factors that may influence follicle growth in vitro.Reproduction (Cambridge, England) · 2024Article
- Multi-cellular engineered living systems to assess reproductive toxicology.Reproductive toxicology (Elmsford, N.Y.) · 2024Review
- Three-Dimensionally Printed Agarose Micromold Supports Scaffold-Free Mouse Ex Vivo Follicle Growth, Ovulation, and Luteinization.Bioengineering (Basel, Switzerland) · 2024Article
- Shear wave elastography to assess stiffness of the human ovary and other reproductive tissues across the reproductive lifespan in health and disease†.Biology of reproduction · 2024Review
- Generation of Tailored Extracellular Matrix Hydrogels for the Study of In Vitro Folliculogenesis in Response to Matrisome-Dependent Biochemical Cues.Bioengineering (Basel, Switzerland) · 2024Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Folliculogenesis is a tightly coordinated process essential for generating a fertilization-competent gamete while also producing gonadal hormones that sustain endocrine function. In vitro follicle growth systems have been critical to our understanding of key events in folliculogenesis, such as gonadotropin-independent and dependent growth, steroid hormone production, and oocyte growth and maturation (cytoplasmic and meiotic). Although there are several successful follicle culture strategies, the following protocol details an encapsulated in vitro follicle growth (eIVFG) system for use with mouse ovarian follicles. Encapsulated IVFG is performed with alginate hydrogels, which are biologically inert, maintains cell-to-cell interactions between granulosa cells and the oocyte, and preserves follicle architecture as found in the ovary. The system supports follicle growth, development, and differentiation from the early primary follicle to the antral follicle stage. Moreover, post-folliculogenesis events including meiotic maturation, ovulation, and luteinization are also supported. Importantly, the culture of secondary follicles has successfully resulted in viable pups after blastocyst transfer. This alginate-based eIVFG system is versatile and has broad applications as a tool for interrogating the fundamental biology of the ovarian follicle in a controlled manner, a screening platform for toxicity and bioactivity, and a potential fertility preservation method for endangered species as well as humans.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.