ArticleAnalytical and bioanalytical chemistry2022
Determination of hair cortisol in horses: comparison of immunoassay vs LC-HRMS/MS.
Article in Analytical and bioanalytical chemistry, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed, 12 citations in OpenAlex.
- Equine Blood and Salivary Cortisol Levels While Exercising on a Water Treadmill with an Artificial River System.Animals : an open access journal from MDPI · 2026Article
- A Validated LC-MS/MS Method for Simultaneous Determination of Cortisol and Cortisone in Grey Wolf Hair for Application in Ecological Studies.Molecules (Basel, Switzerland) · 2026Article
- Donkey hair cortisol concentrations are associated with carrying heavy load and being beaten at work.BMC veterinary research · 2025Article
- Surrogate analyte approach for the determination of endogenous cortisol, cortisone, DHEA, DHEAS in horse hair and sheep wool by LC-HRMS/MS.Scientific reports · 2025Article
- Validation of a Commercial ELISA Kit for Non-Invasive Measurement of Biologically Relevant Changes in Equine Cortisol Concentrations.Animals : an open access journal from MDPI · 2024Article
- A validated LC-MS/MS method for simultaneous determination of key glucocorticoids in animal hair for applications in conservation biology.Scientific reports · 2023Article
- Emerging diagnostic methods and imaging modalities in cushing's syndrome.Frontiers in endocrinology · 2023Review
Corrections and comments
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
Abstract
The measure of hair cortisol concentration (HCC) is becoming an emerging approach to monitor mid-/long-term stress in animals, so it is more and more important to develop accurate and reliable methods. In the light of this, the aim of the present study was to compare mane HCCs of 47 horses with different managements, by means of an immunoassay (ELISA) and liquid chromatography coupled to hybrid high-resolution mass spectrometry (LC-HRMS/MS). After the washing step, the ground hair was extracted with methanol. The extract was evaporated and redissolved in two different aqueous solutions, depending on the detection technique. The methods were validated according to EMA guideline for bioanalytical method validation, in the range 2-50 pg mg
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