Evidence map›Paper›PMID 36136097›Full record

ArticleThe Journal of cell biology2022

ER membrane contact sites support endosomal small GTPase conversion for exosome secretion.

Frederik J Verweij, Maarten P Bebelman, Anna E George, Mickael Couty, Anaïs Bécot, Roberta Palmulli, Xavier Heiligenstein, Julia Sirés-Campos, Graça Raposo, Dirk Michiel Pegtel and 1 more

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of cell biology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.

0numbers the graph read from it
0cells of the map it votes in
51citing papers in PubMed
5.7field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

51 citing papers in PubMed, 70 citations in OpenAlex.

  1. Article
  2. Review
  3. Rab27: Molecular switch of tumor exosome secretion (Review).International journal of molecular medicine · 2026
    Review
  4. Review
  5. Article
  6. Review
  7. Article
  8. Review
  9. Article
  10. Article
  11. Review
  12. Review
  13. Review
  14. Article
  15. Article
  16. Review
  17. Review
  18. Review
  19. Article
  20. Review
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

11 authors at 4 institutions in 2 countries.

Frederik J VerweijInstitute for Psychiatry and Neurosciences of Paris, Hopital Saint-Anne, Université de Paris, Institut national de la santé et de la recherche médicale, U1266, Paris, France.ORCID 0000-0001-8879-1174
Maarten P Bebelman *Institute for Psychiatry and Neurosciences of Paris, Hopital Saint-Anne, Université de Paris, Institut national de la santé et de la recherche médicale, U1266, Paris, France.ORCID 0000-0003-4007-5093
Anna E George *Department of Cell Biology, Neurobiology and Biophysics, Utrecht University, Utrecht, The Netherlands.ORCID 0000-0001-7009-1057
Mickael CoutyInstitute for Psychiatry and Neurosciences of Paris, Hopital Saint-Anne, Université de Paris, Institut national de la santé et de la recherche médicale, U1266, Paris, France.ORCID 0000-0003-0994-5900
Anaïs BécotInstitute for Psychiatry and Neurosciences of Paris, Hopital Saint-Anne, Université de Paris, Institut national de la santé et de la recherche médicale, U1266, Paris, France.
Roberta PalmulliInstitute for Psychiatry and Neurosciences of Paris, Hopital Saint-Anne, Université de Paris, Institut national de la santé et de la recherche médicale, U1266, Paris, France.ORCID 0000-0003-2271-3186
Xavier HeiligensteinInstitut Curie, Paris Sciences & Lettres Research University, CNRS, UMR144, Paris, France.
Julia Sirés-CamposInstitut Curie, Paris Sciences & Lettres Research University, CNRS, UMR144, Paris, France.
Graça RaposoInstitut Curie, Paris Sciences & Lettres Research University, CNRS, UMR144, Paris, France.ORCID 0000-0001-9590-1298
Dirk Michiel PegtelDepartment of Pathology, Cancer Center Amsterdam, Amsterdam University Medical Center, Amsterdam, The Netherlands.ORCID 0000-0002-7357-4406
Guillaume van NielInstitute for Psychiatry and Neurosciences of Paris, Hopital Saint-Anne, Université de Paris, Institut national de la santé et de la recherche médicale, U1266, Paris, France.ORCID 0000-0002-8651-9705
Université Paris Cité · FRCentre National de la Recherche Scientifique · FRUtrecht University · NLAmsterdam University Medical Centers · NL

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Exosomes are endosome-derived extracellular vesicles involved in intercellular communication. They are generated as intraluminal vesicles within endosomal compartments that fuse with the plasma membrane (PM). The molecular events that generate secretory endosomes and lead to the release of exosomes are not well understood. We identified a subclass of non-proteolytic endosomes at prelysosomal stage as the compartment of origin of CD63 positive exosomes. These compartments undergo a Rab7a/Arl8b/Rab27a GTPase cascade to fuse with the PM. Dynamic endoplasmic reticulum (ER)-late endosome (LE) membrane contact sites (MCS) through ORP1L have the distinct capacity to modulate this process by affecting LE motility, maturation state, and small GTPase association. Thus, exosome secretion is a multi-step process regulated by GTPase switching and MCS, highlighting the ER as a new player in exosome-mediated intercellular communication.

Indexed as

Endoplasmic ReticulumEndosomesExosomesrab GTP-Binding ProteinsBiological TransportCell CommunicationCell Membranerab GTP-Binding Proteins

Identifiers

PMID36136097
PMCPMC9507465
OpenAlexW4296793399

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-SA
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.