ArticlePlant physiology2022
Clay nanoparticles efficiently deliver small interfering RNA to intact plant leaf cells.
Article in Plant physiology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 55 citations in OpenAlex.
- Foliar and root absorption of LDH nanosheets loaded with dsRNAs efficiently silenced the expression of whitefly Bemisia tabaci Laccase 1 and Vitellogenin Receptor genes for their effective control.Biotechnology letters · 2026Article
- The unfulfilled potential of nanocarriers for RNA delivery in antiviral crop protection.Nature plants · 2026Review
- Molecular Targeted Suppression of Male Fertility in Amaranthus palmeri S. Watson: Function and Layered Double Hydroxide Nanosheets-Based Delivery System of ApmiR319.Plant biotechnology journal · 2026Article
- Genetic Transformation of Catharanthus roseus with Simplified Nanocarrier-Based Gene Delivery Method Using Green Synthesized Superparamagnetic Iron Oxide Nanoparticles.Molecular biotechnology · 2026Article
- Spray-induced gene silencing for plant disease control: mechanistic basis and deployment-oriented design.Frontiers in plant science · 2026Review
- Programmable nanocarriers for precision plant engineering: converging nanotechnology, CRISPR, and next-generation breeding.Frontiers in plant science · 2026Review
- Nanoparticle-Mediated Nucleic Acid Delivery Systems in Plant Biotechnology: Recent Advances and Emerging Challenges.Plants (Basel, Switzerland) · 2025Review
- Nanotechnology Strategies in Plant Genetic Engineering: Intelligent Delivery and Precision Editing.Plants (Basel, Switzerland) · 2025Review
- RNAi technology development for weed control: all smoke and no fire?Pest management science · 2025Review
- Advancing cancer gene therapy: the emerging role of nanoparticle delivery systems.Journal of nanobiotechnology · 2025Review
- CONSTANS-like 13 homologs MiCOL13 A and MiCOL13B orchestrate flowering time and salt-drought tolerance in mango.Planta · 2025Article
- Trans-Kingdom sRNA Silencing inPathogens (Basel, Switzerland) · 2025Review
- Nanocarrier foliar uptake pathways affect delivery of active agents and plant physiological response.Environmental science. Nano · 2025Article
- Lysozyme-coated nanoparticles for active uptake and delivery of synthetic RNA and plasmid-encoded genes in plants.Nature plants · 2025Article
- Silencing of theInternational journal of molecular sciences · 2024Article
- Nanoparticle LDH enhances RNAi efficiency of dsRNA in piercing-sucking pests by promoting dsRNA stability and transport in plants.Journal of nanobiotechnology · 2024Article
- Exogenously applied silver nanoparticles (AgNPs) differentially affect bacterial blight disease control in twenty-seven wheat cultivars.BMC plant biology · 2024Article
- Engineered dsRNA-protein nanoparticles for effective systemic gene silencing in plants.Horticulture research · 2024Article
- Unlocking the potential of nanoscale sulfur in sustainable agriculture.Chemical science · 2024Review
- Nanoparticle-mediated dsRNA delivery for precision insect pest control: a comprehensive review.Molecular biology reports · 2024Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
RNA interference is triggered in plants by the exogenous application of double-stranded RNA or small interfering RNA (siRNA) to silence the expression of target genes. This approach can potentially provide insights into metabolic pathways and gene function and afford plant protection against viruses and other plant pathogens. However, the effective delivery of biomolecules such as siRNA into plant cells is difficult because of the unique barrier imposed by the plant cell wall. Here, we demonstrate that 40-nm layered double hydroxide (LDH) nanoparticles are rapidly taken up by intact Nicotiana benthamiana leaf cells and by chloroplasts, following their application via infiltration. We also describe the distribution of infiltrated LDH nanoparticles in leaves and demonstrate their translocation through the apoplast and vasculature system. Furthermore, we show that 40-nm LDH nanoparticles can greatly enhance the internalization of nucleic acids by N. benthamiana leaf cells to facilitate siRNA-mediated downregulation of targeted transgene mRNA by >70% within 1 day of exogenous application. Together, our results show that 40-nm LDH nanoparticle is an effective platform for delivery of siRNA into intact plant leaf cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.