ArticleJournal of fungi (Basel, Switzerland)2022
Next-Generation Sequencing of Ancient and Recent Fungarium Specimens.
Article in Journal of fungi (Basel, Switzerland), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- Article
- Whole genome sequencing of historical specimens from the world's largest fungal collection yields high-quality assemblies.The New phytologist · 2026Article
- Phylogeny ofPersoonia · 2026Article
- From herbarium to life: Implications of reviving historical fungi for modern plant pathology and agriculture.iScience · 2025Article
- Article
- Article
- Does One Size Fit All? Variations in the DNA Barcode Gaps of Macrofungal Genera.Journal of fungi (Basel, Switzerland) · 2023Article
- Applying a modified metabarcoding approach for the sequencing of macrofungal specimens from fungarium collections.Applications in plant sciencesArticle
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3 authors.
Funding
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Abstract
Fungaria are an unmatched resource for providing genetic data from authoritative, taxonomically-correct fungal species, especially type specimens. These specimens serve to anchor species hypotheses by enabling the correct taxonomic placement of taxa in systematic studies. The DNA from ancient specimens older than 30 years is commonly fragmented, and sometimes highly contaminated by exogenous, non-target fungal DNA, making conventional PCR amplification and Sanger sequencing difficult or impossible. Here, we present the results of DNA extraction, PCR amplification of the ITS2 region, and Illumina MiSeq Nano sequencing of nine recent and 11 ancient specimens, including seven type specimens. The taxa sampled included a range of large and fleshy, to small and tough, or small, melanized specimens of
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