Evidence map›Paper›PMID 36135182›Full record

ArticleCurrent issues in molecular biology2022

Different N-Glycosylation Sites Reduce the Activity of Recombinant DSPAα2.

Huakang Peng, Mengqi Wang, Nan Wang, Caifeng Yang, Wenfang Guo, Gangqiang Li, Sumei Huang, Di Wei, Dehu Liu

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Current issues in molecular biology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.3field-weighted citation impact, top 48% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 4 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 1 country.

Huakang PengBiotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China.ORCID 0000-0003-3991-9375
Mengqi WangBiotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
Nan WangBiotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
Caifeng YangBiotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
Wenfang GuoBiotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
Gangqiang LiBiotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
Sumei HuangBiotechnology Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, China.
Di WeiBiotechnology Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, China.
Dehu LiuBiotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
Chinese Academy of Agricultural Sciences · CNGuangxi Academy of Agricultural Science · CN

Funding

Collaborative Innovation Project of Science and Technology of CAAS-GXSSA CAAS-GXAAS-XTCX2019026-1"Molecular design and breeding of important economic crops in Guangxi Province" and Foundation of Hainan Yazhou Bay Seed Lab B21HJ0212National Natural Science Foundation of China 31901661
6 · The paper itself

Abstract

Bat plasminogen activators α2 (DSPAα2) has extremely high medicinal value as a powerful natural thrombolytic protein. However, wild-type DSPAα2 has two N-glycosylation sites (N185 and N398) and its non-human classes of high-mannose-type N-glycans may cause immune responses in vivo. By mutating the N-glycosylation sites, we aimed to study the effect of its N-glycan chain on plasminogen activation, fibrin sensitivity, and to observe the physicochemical properties of DSPAα2. A logical structure design was performed in this study. Four single mutants and one double mutant were constructed and expressed in

Indexed as

DSPAα2fibrin sensitivityN-glycosylation sitePichia pastorisplasminogen activator

Identifiers

PMID36135182
PMCPMC9497888
OpenAlexW4294142642

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.