Evidence map›Paper›PMID 36134006›Full record

ArticleNanoscale advances2020

Resolving the internal morphology of core-shell microgels with super-resolution fluorescence microscopy.

Pia Otto, Stephan Bergmann, Alice Sandmeyer, Maxim Dirksen, Oliver Wrede, Thomas Hellweg, Thomas Huser

Abstract read
In one paragraph

Article in Nanoscale advances, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Pia OttoPhysical and Biophysical Chemistry, Bielefeld University Germany thomas.hellweg@uni-bielefeld.de.ORCID https://orcid.org/0000-0003-4238-5570
Stephan BergmannBiomolecular Photonics, Bielefeld University Germany thomas.huser@physik.uni-bielefeld.de.
Alice SandmeyerBiomolecular Photonics, Bielefeld University Germany thomas.huser@physik.uni-bielefeld.de.
Maxim DirksenPhysical and Biophysical Chemistry, Bielefeld University Germany thomas.hellweg@uni-bielefeld.de.
Oliver WredePhysical and Biophysical Chemistry, Bielefeld University Germany thomas.hellweg@uni-bielefeld.de.ORCID https://orcid.org/0000-0002-0020-1334
Thomas HellwegPhysical and Biophysical Chemistry, Bielefeld University Germany thomas.hellweg@uni-bielefeld.de.ORCID https://orcid.org/0000-0002-2394-5846
Thomas HuserBiomolecular Photonics, Bielefeld University Germany thomas.huser@physik.uni-bielefeld.de.ORCID https://orcid.org/0000-0003-2348-7416

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

We investigate the internal morphology of smart core-shell microgels by super-resolution fluorescence microscopy exploiting a combination of 3D single molecule localization and structured illumination microscopy utilizing freely diffusing fluorescent dyes. This approach does not require any direct chemical labeling and does not perturb the network structure of these colloidal gels. Hence, it allows us to study the morphology of the particles with very high precision. We found that the structure of the core-forming seed particles is drastically changed by the second synthesis step necessary for making the shell, resulting in a core region with highly increased dye localization density. The present work shows that super-resolution microscopy has great potential with respect to the study of soft colloidal systems.

Identifiers

PMID36134006
PMCPMC9416983

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.