Evidence map›Paper›PMID 36129255›Full record

ArticleThe journal of histochemistry and cytochemistry : official journal of the Histochemistry Society2022

Effect of Antigen Retrieval on Genomic DNA From Immunodissected Samples.

Donald J Johann, Ik Jae Shin, Adam Roberge, Sarah Laun, Erich A Peterson, Meei Liu, Matthew A Steliga, Jason Muesse, Michael R Emmert-Buck, Michael A Tangrea

Open access · greenAbstract read
In one paragraph

Article in The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.3field-weighted citation impact, top 44% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 4 citations in OpenAlex.

  1. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 4 institutions in 1 country.

Donald J JohannWinthrop P. Rockefeller Cancer Institute, University of Arkansas for Medical Sciences, Little Rock, Arkansas.
Ik Jae ShinWinthrop P. Rockefeller Cancer Institute, University of Arkansas for Medical Sciences, Little Rock, Arkansas.
Adam RobergeAvoneaux Medical Institute, Baltimore, Maryland.
Sarah LaunAvoneaux Medical Institute, Baltimore, Maryland.
Erich A PetersonWinthrop P. Rockefeller Cancer Institute, University of Arkansas for Medical Sciences, Little Rock, Arkansas.
Meei LiuWinthrop P. Rockefeller Cancer Institute, University of Arkansas for Medical Sciences, Little Rock, Arkansas.
Matthew A SteligaWinthrop P. Rockefeller Cancer Institute, University of Arkansas for Medical Sciences, Little Rock, Arkansas.ORCID 0000-0002-4319-5798
Jason MuesseWinthrop P. Rockefeller Cancer Institute, University of Arkansas for Medical Sciences, Little Rock, Arkansas.ORCID 0000-0003-4812-8866
Michael R Emmert-BuckAvoneaux Medical Institute, Baltimore, Maryland.
Michael A TangreaAlvin & Lois Lapidus Cancer Institute, Sinai Hospital of Baltimore, LifeBridge Health, Baltimore, Maryland.
Winthrop Rockefeller Foundation · USMaryland Medical Research Institute · USLoyola University Maryland · USSinai Hospital · US

Funding

Development and Validation of a Microdissection Method to Advance Precision Medicine in the Clinical SettingR33CA202811 · NCI · UNIV OF ARKANSAS FOR MED SCIS · PI JOHANN, DONALD, TANGREA, MICHAEL · 2016 to 2018
$1.2M
NCI NIH HHS R33 CA202811
6 · The paper itself

Abstract

Immunohistochemical (IHC) staining is an established technique for visualizing proteins in tissue sections for research studies and clinical applications. IHC is increasingly used as a targeting strategy for procurement of labeled cells via tissue microdissection, including immunodissection, computer-aided laser dissection (CALD), expression microdissection (xMD), and other techniques. The initial antigen retrieval (AR) process increases epitope availability and improves staining characteristics; however, the procedure can damage DNA. To better understand the effects of AR on DNA quality and quantity in immunodissected samples, both clinical specimens (

Indexed as

Lung NeoplasmsProto-Oncogene Proteins p21(ras)AntigensDNAEpitopesGenomicsHumansMutationPeptide HydrolasesAntigensDNAEpitopesPeptide HydrolasesProto-Oncogene Proteins p21(ras)antigen retrievalDNAmicrodissection

Identifiers

PMID36129255
PMCPMC9527476
OpenAlexW4296714960

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.