Evidence map›Paper›PMID 36125764›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2022

Methods to Analyze Motility in Eury- and Crenarchaea.

Megha Patro, Marleen van Wolferen, Xing Ye, Sonja-Verena Albers, Tessa E F Quax

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Megha PatroMolecular Biology of Archaea, Faculty of Biology, Institute of Biology II, University of Freiburg, Freiburg, Germany.
Marleen van WolferenMolecular Biology of Archaea, Faculty of Biology, Institute of Biology II, University of Freiburg, Freiburg, Germany.
Xing YeMolecular Biology of Archaea, Faculty of Biology, Institute of Biology II, University of Freiburg, Freiburg, Germany.
Sonja-Verena AlbersMolecular Biology of Archaea, Faculty of Biology, Institute of Biology II, University of Freiburg, Freiburg, Germany. sonja.albers@biologie.uni-freiburg.de.
Tessa E F QuaxArchaeal Virus-Host Interactions, Faculty of Biology, Institute of Biology II, University of Freiburg, Freiburg, Germany. t.e.f.quax@rug.nl.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Many archaea display swimming motility in liquid medium, which is empowered by the archaellum. Directional movement requires a functional archaellum and a sensing system, such as the chemotaxis system that is used by Euryarchaea. Two well-studied models are the euryarchaeon Haloferax volcanii and the crenarchaeon Sulfolobus acidocaldarius. In this chapter we describe two methods to analyze their swimming behavior and directional movement: (a) time-lapse microscopy under native temperatures and (b) spotting on semi-solid agar or gelrite plates. Whereas the first method allows for deep analysis of swimming behavior, the second method is suited for high throughput comparison of multiple strains.

Indexed as

Archaeal ProteinsHaloferax volcaniiSulfolobus acidocaldariusAgarChemotaxisAgarArchaeal ProteinsArchaeaArchaellumHaloferaxMotilitySemi-solid agar platesSulfolobusThermomicroscopyTime-lapse microscopy

Identifiers

PMID36125764

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.