Evidence map›Paper›PMID 36125753›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2022

Conversion of Ribosome-Protected mRNA to DNA and Deep Sequencing for Ribosome Profiling in Haloferax volcanii.

Diego Rivera Gelsinger, Emma Dallon, Jocelyne DiRuggiero

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Diego Rivera GelsingerDepartment of Biochemistry and Molecular Biophysics, Columbia University, New York, NY, USA.
Emma DallonJohns Hopkins University, Department of Biology, Johns Hopkins University, Baltimore, MD, USA.
Jocelyne DiRuggieroJohns Hopkins University, Department of Biology, Johns Hopkins University, Baltimore, MD, USA. jdiruggiero@jhu.edu.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The translation of messenger RNA (mRNA) into protein is an essential process for all forms of life. The ability to monitor this process in a quantitative way by ribosome profiling-based approaches has revolutionized our ability to monitor protein synthesis in vivo and to explore and model complex cellular processes. Ribosome profiling is a high-throughput technique that globally analyzes the full set of ribosomes engaged in translation, providing insights into important aspects of the mechanism of protein synthesis and its regulation. This protocol covers the construction of a ribosome profiling library from culture harvesting, footprint isolation via ultracentrifugation, gel-based size fractionation, and footprint sequencing for a model halophilic archaeon, Haloferax volcanii. This approach has revealed the first global view of translation in the archaea.

Indexed as

Haloferax volcaniiDNAHigh-Throughput Nucleotide SequencingProtein BiosynthesisRibosomesRNA, MessengerDNARNA, MessengerArchaeaFootprintHigh throughput sequencingRibosomeTranslation efficiency

Identifiers

PMID36125753

What OpenQuestion holds

Textmetadata
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.