Evidence map›Paper›PMID 36125716›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2022

Live-cell Imaging of Biosynthetic Protein Transport in Hepatocytes.

Francisco Lázaro-Diéguez, Anne Müsch

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact, top 53% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 0 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 1 institution in 1 country.

Francisco Lázaro-DiéguezDepartment of Developmental and Molecular Biology, Albert Einstein College of Medicine, Bronx, NY, USA. francisco.lazaro-dieguez@einsteinmed.edu.
Anne MüschDepartment of Developmental and Molecular Biology, Albert Einstein College of Medicine, Bronx, NY, USA.
Albert Einstein College of Medicine · US

Funding

Cell-cell adhesion-mediated signaling determines epithelial polarization in the liverR01DK118015 · NIDDK · ALBERT EINSTEIN COLLEGE OF MEDICINE · PI MUESCH, ANNE · 2019 to 2024
$3.0M
NIDDK NIH HHS R01 DK118015
6 · The paper itself

Abstract

Here, we describe a strategy to analyze the exit of apical and basolateral cargo from the trans-Golgi network in primary hepatocytes. The method is based on recombinant adenovirus-mediated infection combined with a pulse-chase regimen and live-cell imaging analysis of fluorescent protein-tagged dipeptidyl peptidase IV (DPPIV) and vesicular stomatitis virus G (VSVG) cargo proteins, coexpressed and accumulated in the endoplasmic reticulum via DPPIV aggregation through an engineered conditional aggregation domain and VSVG by exploiting the aggregation of the ts045 mutant at its non-permissive temperature of 40 °C.

Indexed as

Dipeptidyl Peptidase 4trans-Golgi NetworkHepatocytesProtein TransportRecombinant ProteinsDipeptidyl Peptidase 4Recombinant ProteinsApical cargoConditional aggregation domainHepatocyteLive-cell imagingProtein sortingProtein targetingProtein transporttrans-Golgi network

Identifiers

PMID36125716
PMCPMC9627190
OpenAlexW4296502173

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.