Evidence map›Paper›PMID 36125674›Full record

ArticleMolecular biology reports2022

Pangenomes-identified singletons for designing specific primers to identify bacterial strains in a plant growth-promoting consortium.

Roel Alejandro Chávez-Luzanía, Amelia C Montoya-Martínez, Fannie Isela Parra-Cota, Sergio de Los Santos-Villalobos

Abstract read
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In one paragraph

Article in Molecular biology reports, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
1.1field-weighted citation impact, top 19% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 5 citations in OpenAlex.

  1. Review
  2. Plants (Basel, Switzerland) · 2023
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 1 country.

Roel Alejandro Chávez-Luzanía *Instituto Tecnológico de Sonora. 5 de febrero 818 sur, CP. 85000, Cd. Obregón, SON, México.
Amelia C Montoya-Martínez *Instituto Tecnológico de Sonora. 5 de febrero 818 sur, CP. 85000, Cd. Obregón, SON, México.
Fannie Isela Parra-CotaCampo Experimental Norman E. Borlaug, Instituto Nacional de Investigaciones forestales, Agrícolas y Pecuarias, Norman E. Borlaug Km. 12, C.P. 85000, Ciudad Obregón, SON, México.
Sergio de Los Santos-VillalobosInstituto Tecnológico de Sonora. 5 de febrero 818 sur, CP. 85000, Cd. Obregón, SON, México. sergio.delossantos@itson.edu.mx.ORCID http://orcid.org/0000-0003-2234-7147
Sonora Institute of Technology · MXInstituto Nacional de Investigaciones Forestales Agrícolas y Pecuarias · MX

Funding

ITSON PROFAPI 2022_0001
6 · The paper itself

Abstract

backgroundThe use of plant growth-promoting microorganisms represents a sustainable way to increase agricultural yields and plant health. Thus, the identification and tracking of these microorganisms are determinants for validating their positive effects on crops. Pangenomes allow the identification of singletons that can be used to design specific primers for the detection of the studied strains.

objectiveThis study aimed to establish a strategy based on the use of whole-genome sequencing and pangenomes for designing and validating primer sets for detecting Bacillus cabrialesii TE3 METHODS AND

resultsThe identification of singletons of TE3

conclusionsThe use of pangenomes allowed the distinction of unique sequences that enables the design of primers for specific identification of the studied bacterial strains. This strategy can be widely used for the design of primer sets to detect other strains of interest for combating biopiracy, and commercial protection of biological products, among other applications.

Indexed as

BacteriaPlant DevelopmentCrops, AgriculturalTriticumWhole Genome SequencingBacillus cabrialesiiBacillus paralicheniformisPGPRPriestia megateriumPrimer design

Identifiers

PMID36125674
OpenAlexW4296391931

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.