Evidence map›Paper›PMID 36125659›Full record

ArticleLasers in medical science2022

Effects of different protocols of defocused high-power laser on the viability and migration of myoblasts-a comparative in vitro study.

Fernanda Thomé Brochado, Belkiss Câmara Mármora, Paloma Santos Campos, Tuany Rafaeli Schmidt, Kristianne Porta Santos Fernandes, Sandra Kalil Bussadori, Lucas Gonçalves Santos, Vivian Petersen Wagner, Marcelo Lazzaron Lamers, Manoela Domingues Martins

Abstract read
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In one paragraph

Article in Lasers in medical science, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Fernanda Thomé BrochadoDepartment of Oral Pathology, School of Dentistry, Faculdade de Odontologia, Federal University of Rio Grande Do Sul, Rua Ramiro Barcelos, CEP, sala 503, Porto Alegre, RS, 249290035-003, Brazil.
Belkiss Câmara MármoraDepartment of Oral Pathology, School of Dentistry, Faculdade de Odontologia, Federal University of Rio Grande Do Sul, Rua Ramiro Barcelos, CEP, sala 503, Porto Alegre, RS, 249290035-003, Brazil.
Paloma Santos CamposDepartment of Oral Pathology, School of Dentistry, Faculdade de Odontologia, Federal University of Rio Grande Do Sul, Rua Ramiro Barcelos, CEP, sala 503, Porto Alegre, RS, 249290035-003, Brazil.
Tuany Rafaeli SchmidtDepartment of Oral Pathology, School of Dentistry, Faculdade de Odontologia, Federal University of Rio Grande Do Sul, Rua Ramiro Barcelos, CEP, sala 503, Porto Alegre, RS, 249290035-003, Brazil.
Kristianne Porta Santos FernandesPostgraduation Program in Biophotonics Applied to Health Sciences, Nove de Julho University - UNINOVE, São Paulo, SP, Brazil.
Sandra Kalil BussadoriPostgraduation Program in Biophotonics Applied to Health Sciences, Nove de Julho University - UNINOVE, São Paulo, SP, Brazil.
Lucas Gonçalves SantosDepartment of Oral Pathology, School of Dentistry, Faculdade de Odontologia, Federal University of Rio Grande Do Sul, Rua Ramiro Barcelos, CEP, sala 503, Porto Alegre, RS, 249290035-003, Brazil.
Vivian Petersen WagnerAcademic Unit of Oral and Maxillofacial Medicine and Pathology, Department of Clinical Dentistry, University of Sheffield, Sheffield, UK.
Marcelo Lazzaron LamersDepartment of Oral Pathology, School of Dentistry, Faculdade de Odontologia, Federal University of Rio Grande Do Sul, Rua Ramiro Barcelos, CEP, sala 503, Porto Alegre, RS, 249290035-003, Brazil.
Manoela Domingues MartinsDepartment of Oral Pathology, School of Dentistry, Faculdade de Odontologia, Federal University of Rio Grande Do Sul, Rua Ramiro Barcelos, CEP, sala 503, Porto Alegre, RS, 249290035-003, Brazil. manomartins@gmail.com.ORCID http://orcid.org/0000-0001-8662-5965

Funding

Hospital de Clínicas de Porto Alegre 2018-0624
6 · The paper itself

Abstract

The aim of the present study was to analyze for the first time the effect of photobiomodulation therapy (PBMT) using defocused high-power laser (DHPL) in myoblast cell line C2C12 viability and migration and compare them with low-power laser therapy. Cells were divided into 9 groups: Sham irradiation 10% fetal bovine serum (FBS); Sham irradiation 5%FBS; low-power laser 0.1 W; DHPL 810 1 W; DHPL 810 2 W; DHPL 980 1 W; DHPL 980 2 W; DHPL dual 1 W; DHPL dual 2 W. To simulate stress conditions, all groups exposed to irradiation were maintained in DMEM 5% FBS. The impact of therapies on cell viability was assessed through sulforhodamine B assay and on cells migration through scratch assays and time-lapse. Myoblast viability was not modified by PBMT protocols. All PBMT protocols were able to accelerate the scratch closure after 6 and 18 h of the first irradiation (p < 0.001). Also, an increase in migration speed, with a more pronounced effect of DHPL laser using dual-wavelength protocol with 2 W was observed (p < 0.001). In conclusion, the diverse PBMT protocols used in this study accelerated the C2C12 myoblasts migration, with 2-W dual-wavelength outstanding as the most effective protocol tested. Benefits from treating muscle injuries with PBMT appear to be related to its capacity to induce cell migration without notable impact on cell viability.

Indexed as

Low-Level Light TherapyMyoblastsCell MovementCell SurvivalLasersHigh-power laser therapyLow-power laser therapyMuscle regenerationMuscle repairPhotobiomodulation therapy

Identifiers

PMID36125659

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.