Robust neuronal differentiation of human embryonic stem cells for neurotoxicology.

Athina Samara, Martin Falck, Mari Spildrejorde, Magnus Leithaug, Ganesh Acharya, Robert Lyle, Ragnhild Eskeland

Abstract read

In one paragraph

Article in STAR protocols, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it

0cells of the map it votes in

3citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

Citalopram exposure of hESCs during neuronal differentiation identifies dysregulated genes involved in neurodevelopment and depression.Frontiers in cell and developmental biology · 2024
Article
ANDA: an open-source tool for automated image analysis of in vitro neuronal cells.BMC neuroscience · 2023
Article
Multi-omics approach reveals dysregulated genes during hESCs neuronal differentiation exposure to paracetamol.iScience · 2023
Article

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Athina SamaraDivision of Clinical Paediatrics, Department of Women's and Children's Health, Karolinska Institutet, 17177 Stockholm, Sweden; Astrid Lindgren Children's Hospital Karolinska University Hospital, 17177 Stockholm, Sweden. Electronic address: athina.samara@ki.se.

Martin FalckDepartment of Biosciences, University of Oslo, Blindern, PO Box 1066, 0316 Oslo, Norway; PharmaTox Strategic Research Initiative, Faculty of Mathematics and Natural Sciences, University of Oslo, 0316 Oslo, Norway.

Mari SpildrejordePharmaTox Strategic Research Initiative, Faculty of Mathematics and Natural Sciences, University of Oslo, 0316 Oslo, Norway; Department of Medical Genetics and Norwegian Sequencing Centre, Oslo University Hospital, Kirkeveien 166, 0450 Oslo, Norway; Institute of Clinical Medicine, Faculty of Medicine, University of Oslo, 0450 Oslo, Norway.

Magnus LeithaugPharmaTox Strategic Research Initiative, Faculty of Mathematics and Natural Sciences, University of Oslo, 0316 Oslo, Norway; Department of Medical Genetics and Norwegian Sequencing Centre, Oslo University Hospital, Kirkeveien 166, 0450 Oslo, Norway.

Ganesh AcharyaDivision of Obstetrics and Gynecology, Department of Clinical Science, Intervention and Technology (CLINTEC), Karolinska Institutet, Alfred Nobels Allé 8, 14152 Stockholm, Sweden; Center for Fetal Medicine, Karolinska University Hospital Huddinge, 14186 Stockholm, Sweden.

Robert LylePharmaTox Strategic Research Initiative, Faculty of Mathematics and Natural Sciences, University of Oslo, 0316 Oslo, Norway; Department of Medical Genetics and Norwegian Sequencing Centre, Oslo University Hospital, Kirkeveien 166, 0450 Oslo, Norway; Centre for Fertility and Health, Norwegian Institute of Public Health, PO 222 Skøyen, 0213 Oslo, Norway.

Ragnhild EskelandPharmaTox Strategic Research Initiative, Faculty of Mathematics and Natural Sciences, University of Oslo, 0316 Oslo, Norway; Institute of Basic Medical Sciences, Department of Molecular Medicine, Faculty of Medicine, University of Oslo, Blindern, PO Box 1112, 0317 Oslo, Norway. Electronic address: ragnhild.eskeland@medisin.uio.no.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Here, we describe a protocol for rapid neuronal differentiation from human embryonic stem cells (hESCs) toward a heterogenous population of telencephalic progenitors, immature and mature neurons, for drug-screening and early-brain differentiation studies. hESC neuronal differentiation depends on adhesion and minimal cell-passaging to avert monolayer cross-connectivity rupture. In this protocol, we detail optimized cell-seeding densities and coating conditions with high cell viability suitable for neurotoxicology and high-resolution single-cell omics studies. Daily media changes reduce compound instability and degradation for optimal screening. For complete details on the use and execution of this protocol, please refer to Samara et al. (2022).

Indexed as

Human Embryonic Stem CellsCell DifferentiationCell SurvivalEmbryonic Stem CellsHumansNeuronsCell cultureCell DifferentiationDevelopmental biologyGene ExpressionNeuroscienceStem Cells

Identifiers

PMID36123835

DOI10.1016/j.xpro.2022.101533

PMCPMC9485591

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.